I see 2 random bands in my ERK/pERK blot? Has anyone else seen this?

I ran a western blot using 12% gel and probing for ERK/pERK (cell signaling antibodies). The green is total ERK, the Red is supposed to be phospho-ERK. I see 2 bands below the total ERK that...

22 February 2021 1,777 3 View

Best way to add streptavidin onto flat surface?

I will be adding streptavidin onto the surface of a graphene coated glass slide; thereafter, I will be analyzing with a MALDI-TOF. I was wondering the best way to go about this? I was thinking of...

07 September 2020 5,902 4 View

How to choose Object Classification in Live Video Feed when given multiple high-confidence classifications?

Hello, I am searching for literature on methods employed for choosing object classification when multiple object classifications (e.g. labels) are given with relatively high-confidence during...

21 April 2020 2,564 2 View

Estimate bacterial cells using qpcr?

I have DNA extracted from air filters and wish to use qPCR to estimate the bacterial load. I have read through some papers but am stuck with some methodology. I know I need to make a standard...

02 April 2019 7,476 2 View

What is the extinction coefficient for 300nm Europium particles?

I'm looking for an extinction coefficient for 300nm Europium particles. I've seen a coefficient of 207.2 for 200nm particles but nothing for 300nm.

12 February 2019 2,650 1 View

Looking for suggestions for improving the order of protein crystals grown with Pentaerythritol propoxylate?

Is there anything about a protein that I can learn from the fact that it crystallizes in 35% Pentaerythritol propoxylate. I am working on crystallizing a protein and keep hitting dead ends. I...

16 October 2018 10,021 2 View

Common analytical challenges in industrial biotechnology?

14 September 2017 7,429 3 View

When running a PERMANOVA in PAST using landmark data (geometric morphometrics), which distance should be selected?

My model organisms are damselflies and I landmark the wings.

02 September 2017 3,199 1 View

Is there a distinction between grief response and coping strategies based on proposed grief models?

My thesis involves looking at grief responses based on the shared relationship the bereaved had with the deceased. one of my questions deal with coping strategies but it seems the models do not...

20 April 2017 8,794 7 View

Looking for metabarcoding primers for Asian freshwater macro-invertebrates including freshwater shrimp?

If anyone can point me towards references, it would be greatly appreciated.

12 December 2016 8,995 3 View

How to delaminate copper in water-based anodes?

Hello everyone, I'm looking for a way to delaminate copper current collector of water-based anodes in Li-ion batteries without damaging the graphite film. I tried different materials such as HNO3...

24 February 2021 3,785 3 View

Is anyone working on a project focused on immigrants/newcomers working in the hospitality industry and how they have been impacted by COVID?

The on-going Covid-19 pandemic has had devastating economic effects on many industries. However, few have suffered to the same extent as full-service restaurants. Numerous studies have shown that...

24 February 2021 9,496 4 View

What is the optimum time and temperature of blanching and pre-frying potato for frozen fries?

I am helping a local home industry for developing frozen fries from their local farm. I want to know what is the optimum blanching and pre-frying temperature and time for processing. Please also...

23 February 2021 1,616 5 View

How we can measure Kemp % in wool without using of benzene?

Would you please let me to know about the methods for measurement of medullation and Kemp % on animal? My problem is that the wool sampling and sending to laboratory is not possible. So I would...

21 February 2021 7,260 4 View

Where can the fault diagnosis data set of lithium battery be found?

Most of the data sets on the network only contain good information about the voltage and internal resistance of lithium batteries. The data of faulty battery is missing. Who can help me, I will be...

21 February 2021 8,659 1 View

Not able to charge-discharge Li-ion coin cell at higher C rate ?

I am working on cathode material. I have made different Li-ion coin cell against Li metal using commercial available cathode materials such as LCO,LMO, LFP and NMC and also with the lab...

18 February 2021 1,450 3 View

How to successfully revive cells stored at -80 degree C for a year?

I am working on C6 cells along with other neuronal cell lines. We have preserved the cells in 90%FBS and 10% DMSO solution. We are reviving the cells after a year and cells are either not binding...

18 February 2021 4,499 1 View

No title

Hello. I ask for help - I need to get lithium tetraborate (soluble salt) from lithium carbonate and boric acid. In literature, everything seems to be simple - mix, ignite and - it's done. But it...

16 February 2021 9,523 2 View

What are alternatives for B27+ Supplement in neuronal cell culture?

Hello everybody In our iPSC-derived neuronal culture we use Maturationmedium, supplemented with B27+. As Thermo Fisher run out of stock and can deliver earliest in May, I wanted to ask whether...

16 February 2021 3,348 1 View

Differences between sheep blood with Alsever solution vs citrated/defibrinated sheep blood in microbiological media?

I am aware of a study that has compared the use of citrated and defibrinated sheep's blood in microbiological media (Hair Sheep Blood, Citrated or Defibrinated, Fulfills All Req... ), but can...

10 February 2021 5,927 1 View