We are interested in studying K+ dynamics in cortical slices and to do so we will perform extracellular recordings with K+ sensitive electrodes. However, we have got some problems in buildind these electrodes, since the K+ ionophore I (cocktail A) does not stick to the tip after adding KCl (100mM) as back filling solution in one of the barrels.
I have read that some groups first silanize the barrel and then tip-fill with K+ ionophore and KCL. What is the protocol for silanizing K+-sensitive electrodes?
Thank you,
Alba Bellot Saez
Read literature and find the more appropriate one. This is an old procedure used before the discover of patch-clamp technique. In a library you can find many books about the so called "old-electrophysiological techniques".......Forgotten in the mind of younger electrophysiologists.....
Good luck
- Badges
- Science topic
- Similar topics
- Engineering
- Electrical Engineering
- Electrodes