Hi all,
I want to separate S.cerevisiae spores from their asci so that after plating a sample each colony will result from a single spore and not from the four spores in the ascus. Under the microscope I see that after treating S.cerevisiae asci with zymolase/lyticase the spores are still connected. Is it just a matter of zymolase/lyticase concentration (I've been using 500U/mL lyticase) or is there a way to disconnect the spores after this enzymatic treatment.
Thank you
Dear Daniel Melamed ,
porulation of the baker's yeast Saccharomyces cerevisiae is a response to nutrient depletion that allows a single diploid cell to give rise to four stress-resistant haploid spores. The formation of these spores requires a coordinated reorganization of cellular architecture.
Article Ascospore Formation in the Yeast Saccharomyces cerevisiae
Regards,
Shafagat
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