Please describe a simple method for preparation of Alginate Nanoparticle.
preferably by emulsification crosslinking method.
You can prepare alginate nanoparticles using simple ionotropic gelation technique which is used for microsphere preparation. All you need is to optimize process parameters to get the nanosized spheres, i.e. you may employ homogenization or you can use novel techniques like emulsification (following articles would serve good references)
Synthesis and characterization of calcium alginate nanoparticles, sodium homopolymannuronate salt and its calcium nanoparticles, by H. Daemi in Scientia Iranica
Preparation of Calcium Alginate Nanoparticles Using Water-in-Oil (W/O) Nanoemulsions, by Alexandra H. E. Machado et al. in Langmuir
Calcium-Alginate Nanoparticles Formed by Reverse Microemulsion as Gene Carriers by Jin-Oh You et al. in Macromolecular Symposia
Thanks a lot for your reply Imran. I tried the method mentioned in (Preparation of Calcium Alginate Nanoparticles Using Water-in-Oil (W/O) Nanoemulsions, by Alexandra H. E. Machado et al. in Langmuir) but the separation was very difficult. when centrifuged the nanoparticles were pelleted at interface of oil and water. and even after repeated washing the nanoparticles were settling at the top after centrifugation.
Maruthi, first you need to phase separate the oil and water (which will also remove additional surfactant as reverse micelles) as described in Machado et al. and then you would be able to separate the nanoparticles as a dispersion. Then you may try centrifuging the dispersion to settle nanoparticles.
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