What are technical problems working with HCT116 colon cancer cell line ? how is it maintained and cultured to study the anticancer effects of a drug?

Technical problems working with HCT116 compared with other cell line Caco-2 and HT29

07 January 2021 2,067 3 View

How to cite a reference for a literature review?

Someone confuse me, he said that when you have some information you want to use it from reference say X, and the reference X inspired this information from reference say Y, then so, I should cite...

21 September 2020 8,630 3 View

How is the coronavirus affecting your research and teaching?

The coronavirus is affecting our lives in many ways, including conducting field trips and laboratory experiments in research and teaching. In-person classes are now being moved to online, which is...

16 March 2020 9,288 6 View

How can I extract the intensity of electric field maps (E^2 or H^2) in the CST Microwave Studio?

We are looking for a method to extract the power of electric field from the CST Microwave studio simulation. Thanks

14 October 2019 5,769 1 View

How can I determine a metastable phase?

Dear colleagues, Recently, I found a new metastable phase which has not been reported so far. The approximate phase constitution for this metastable phase has been measured by the EDS technique....

17 March 2019 9,725 3 View

In gromacs MD simulation , I run my file with nsteps = 10000000; 2*10000000=20000ps (20ns). Is this correct for 20ns or it should be 10ns ????

I wants to know what's the relationship between nsteps ,ps,ns .How can I calculated exact time for simulation.?????

07 February 2019 8,118 5 View

How can I dry glycerol on nitrocellulose membrane without damaging antibody?

20 April 2017 793 1 View

When should I block my nc membrane with BSA?

I am creating a sample test of a LFA on a nitrocellulose membrane. I have seen some contradicting protocols regarding blocking the membrane so far. Some suggest doing it before I spot the membrane...

16 April 2016 5,456 2 View

At what setting should I sonicate my carbon nanoparticles?

I will be using the M220 or E210 Covaris sonicator. Any insight would be greatly appreciated! I will be sonicating 1 mg of carbon nanoparticles in 0.1 mL of water and then later with added borate...

14 April 2016 245 3 View

How do I create Washing Buffer (5 mM BB, 1% (w/v) BSA and Storage Buffer (Sb, 100 mM BB, 1% (w/v))?

I already have the 5 mM Borate Buffer and the 100 mM Borate Buffer. I also have 10% BSA. How do I create 5 mL of the WB and 5 mL of the SB? I need these two buffers to couple carbon nanoparticles...

12 April 2016 1,659 3 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

flexibility of polymers chain

effect of double bond on the flexibility of polymers

07 April 2021 0 0 View

Formulation of polymer color flakes

I am writing to request some information for making polymer color flakes. I would appreciate the help.

17 March 2021 0 0 View

Polymer composites suddenly solidifies?

I am making polymer composite by solvent mixing method. When I am trying to add filler solution in polymer solution it quickly get solidified. How to make them then uinform? It doesn't give time...

03 March 2021 7,540 4 View

What techniques can I use to determine the miscibility of polymer blends?

The term miscibility refers to the single-phase state in thermodynamics. I do not mean the compatibility of different components. To determine the miscibility I know several techniques such as...

03 March 2021 4,107 4 View