What is the best way to grow Clostridium difficile in the laboratory?

More Rusha Pal's questions See All

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Can we use two antibodies generated in same species for indirect Sandwich ELISA?

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26 October 2020 2,259 7 View

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Can any body suggest the funding agency for Research Project on climate change and Remote Sensing?

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02 March 2021 5,785 3 View

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Samples to study circular dichroism (chirality)?

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How to accurately quantify chitin?

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Can anyone help with Schistosoma identification?

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Suppose we have a banana seller cart consisting of 4 wheels, weighing about 300 kg. How much torque motors are needed to pull of push it electrically?

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How does trust influence the development of environmental projects?

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What are the conflicts between EIA and environmental engineers and lawyers?

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Marcela Krutova

Dear Dr. Rusha Pal

Before culture on selective media for C. diff. I would recommend you to do "alcohol shock" treatment of stool sample (1:1 with 70% methyl alcohol for 30-60 min, room temperature). Then you can inoculate this stool sample (2 drops) directly on CCFA agar (e.g. BRAZIER'S CLOSTRIDIUM DIFFICILE SELECTIVE AGAR ) as was suggested by Dr. Zimmermann as the second step for subculture of C. difficile isolate.

You will save money and time. Chromogenic agar works without "alcohol-shock" but DNA from colonies is not suficient for molecular typing.

Kind regards,

Marcela

Yi-Hsuan Su

Hi Dr. Pal,

If you are planning to grow laboratory C.diff strain, you can use brain heart infusion broth (from BD) at 37 degree in anaerobic chamber. I had a lot of success growing strains from ATCC and clinical isolates.

Best,

Yi-Hsuan