Proper RP-HPLC workup following peptide synthesis?

Hello, I've recently synthesized a short (17aa) peptide via manual SPPS. I was instructed to submit a small pipette tip's worth of material onto a capillary column to obtain a rough estimate of...

12 January 2021 9,432 3 View

SPPS: peptide failure to elongate?

SPPS of a 16 aa peptide was performed on a 0.15 mmol scale. Resin gained a minimal amount of weight: starting weight of 224 mg and a final weight of 280.I believe my peptide failed to elongate but...

21 December 2020 9,428 11 View

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Greetings to all. I have some questions about setting up DFT calculations in Gaussian software (I am a beginner in these matters), and trying to calculate the energy of the first excited triplet...

06 December 2020 3,504 4 View

Problems with fluorescent bioconjugation of short peptide?

I have an 18aa azido-peptide conjugated at the N-terminus (cysteine). Synthesis and folding were validated through HPLC and mass spec. Multiple attempts were made with Dylight-phosphine conjugates...

02 August 2020 2,775 3 View

What is the experimental set up/or protocol to measure distribution of the light emitted?

Hello, Could anyone here please tell me the experimental set up, or the protocol to be able to measure the angular distribution of the light emitted (fD) by some OLED device? I have seen that...

10 February 2020 7,932 2 View

How to measure HRV-PRV agreement using Bland-Altman plots from Excel (step-by-step guide)?

Hi! I recently installed the Real Statistics complement for building Bland-Altman plots in Excel. However, I don't know how to use it in evaluating the agreement between HRV and PRV series. Could...

25 November 2019 3,736 3 View

What is the minimum number of PP intervals required for accurate and reliable extraction of Pulse Rate Variability (PRV) from PPG signals?

The minimum number of RR intervals required for reproduce several metrics derived from HRV (e.g., HF power) can be found in the literature (Richards et al., 2010). However, what about PPG signals...

02 June 2019 353 2 View

Any fast publishing Scopus indexed journal ?

Who can help with a link to the a fast publishing scopus indexes journal. I am looking at a journal that will respond in like 3-5 weeks

19 August 2018 1,327 18 View

Metagenomics databases. Which one choose?

In bioinformatics databases play an important role in the results, whether chimera detection or OTUs assignment (16S metagenomics). In your experience, which databases have a better relation...

11 January 2018 858 3 View

Meshing Advise for 2 phase flow ANSYS FLUENT simulation?

I am having difficulty meshing the attached geometry for a two phase mixing type application where I care about the volume fraction at various points along the tubes. I have defined 3 bodies Body...

05 December 2017 1,306 3 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Why can't make aluminum nitride film successfully by use sputter?

We use reactive sputtering process to make aluminum nitride film, and want to make c-axis aluminum nitride film on a silicon substrate, but the crystal orientation cannot be seen with...

03 March 2021 7,975 3 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

Irradiation data for JAPAN?

Hi, Could you help me to get the latest (2019, 2020) solar resource data for a particular site in JAPAN? PV GIS and NSRDB data are not in reach to JAPAN, I'm looking for some other database for...

03 March 2021 961 3 View

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02 March 2021 3,060 3 View

Why are the velocities so high in my Boussinesq approximation Fluent simulation?

Hello, I have a mechanism where air rises due to convective flows. In this simulation, nothing is driving the air except this. I understand that the Boussinesq approximation only works for small...

02 March 2021 4,910 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View