What is the best way to cryopreserve tumors for later cryosectioning and immunofluorescence?

The cryopreservation protocol I am familiar with is as following: 1. PFA 4% overnight at 4C 2. WashX3 cold PBS 3. Sucrose 15% at 4C until submerged 4. Sucrose 30% at 4C overnight 5. OCT...

06 November 2024 1,742 2 View