How to extract nuclei from OCT frozen tissue?

I am trying to extract intact nuclei from OCT-frozen tumor tissue samples. Does anybody have a protocol for doing so? I need as many intact nuclei as possible for performing ATAC-seq on them.

13 September 2020 8,608 1 View

If anyone have 'IMSD (1995) technical guideline' then please provide me?

National Remote Sensing Agency (1995) IMSD Technical Guidelines, Dept. of Space, Govt. of India, Balanagar, Hyderabad. mail me [email protected]

16 September 2018 6,677 3 View

I have very interesting researches on electricity & internet. How can I submit through online ?

I am a dentist but I have very interesting inventions on the electricity & internet. So how can I publish through online ?

20 June 2018 5,522 3 View

Proteins getting stuck at thr interface of stacking and resolving gel of a 6M urea gel, Why?

I am trying to separate a 40 & 43 kda protein. I run 6M urea sds page for that. But most of the proteins get stuck around the interface of resoving and stacking gel. The small amount of...

19 June 2018 3,189 4 View

There is a question about western blotting in huntington model. emergency?

i created a huntington's disease model using plasmide gfp-q74 and gfp-q23(from addgene) in SHSY5Y cell line. then i made BCA for quantification of huntingtin protein and then i made western...

23 March 2018 5,760 3 View

How to study the levels of Phosphorylated Tight junction proteins like ZO1 and Occludin?

Hi all, I want to check the expression of phophorylated ZO1 and occludin from cell-lysate. However I couldn't get any Antibody commercially for phospho-ZO1 or Phospho-occludin as available for...

07 March 2018 7,984 3 View

Singlet to triplet/quintet tddft?

I have tried to do tddft from singlet to quintet state. by writing "#p TD(NSTATE=20,quintet) pbe1pbe/def2svp scf=(xqc,maxcycles=9000)". and have mentioned spin multiplicity=1, but it's shows...

30 January 2018 8,117 1 View

Can any one suggest me the method of collection of Drosophila eggs in large quantity other than petriplae and funnel assembly

I want to collect the Drosophila eggs and do some enzyme assays. In single petriplae I could get only 300 eggs the protein from which is not sufficient for assay. And can I store it in insect ringer.?

06 June 2017 5,041 3 View

Why there are differences in calculation of Refractive Index of nanomaterial , when calculated from 2 route, from reflactance and transmittance data?

please give theoretical analysis

12 April 2017 1,940 2 View

Hi guys, How can I calculate Relative Fluorescence Intensity (RFI) with flowcytometry?

22 September 2016 3,189 3 View

How to normalise data on OPM package from R Studio ?

Good afternoon, I recently used OmniLog from BIOLOG for my experimentations : I tested the metabolism of different strains on 2 types of plates. I have 16 strains of 3 different groups...

02 March 2021 3,584 1 View

Isolates of Hymenoscyphus fraxineus?

Dear Dr. Cai, my name is Simone Prospero and I work in the team of Phytopathology at the Swiss Federal Institute for Forest Snow and Landscape Research (WSL;...

01 March 2021 1,133 1 View

How to remove the residual PDMS on the silicon mold?

Incompletely cross-linked pdms are difficult to remove.

01 March 2021 798 3 View

Where to find data on trees in tropical agroforestry?

Hi, I'm looking for data (mainly related to management: growth rate, canopy size, soil and climate preferences, etc.) about tropical trees used in tropical agroforestry. Have you ever heard about...

28 February 2021 7,356 8 View

The reason that the culture of white fungi Contamination with green mold(Trichoderma), the culture autoclave at121 for 15 min. What is it?

Is the period to autoclave not enough? The inoculation in a hood and flame and UV and alcohol.

27 February 2021 9,356 3 View

The Shannon's index is inversely proportional to Simpson's index (1-D)?

I'm having a problem with my bloxplot with Shannon's and Simpson's indexs. The value of Shannon for my Community 1 indicating higher diversity (in relation of the Community 2), but have lower...

26 February 2021 3,033 9 View

What is the most suitable medium of ascomycetes fungi ??

What is the most suitable medium of ascomycetes fungi ??

26 February 2021 4,413 1 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View

Is there any one who can provide a donation of HPV detection and genotyping kits in exchange collaboration fors?

Warm greetings My name is Ayichew S. and I am doing my Ph.D. in Medical Microbiology (on Human papillomavirus). At this stage, I have almost completed a clinical sample Cervical swabs)...

25 February 2021 1,660 3 View

Is there any one who can provide a donation of HPV detection and genotyping kits in exchange collaboration forms?

Warm greetings! My name is Ayichew S. and I am doing my Ph.D. in Medical Microbiology focusing on Human papillomavirus). At this stage, I have almost completed a clinical sample (Cervical swabs)...

25 February 2021 3,612 3 View