I have microsat data for multiple fish populations, and I'd like to get the best estimates of effective population size as I possibly can, but my problem is I have no age or size class data. Therefore my dataset contains overlapping generations, and I have no way of discerning what individuals are of what age class. Is there an accepted way to calculate Ne despite this overlap? Most every method I see requires you have age class information or discrete generations. Any help is appreciated.
Thanks!