i want to analyse my plant extraction for first time and measure components of my extraction. i extracted my plant with methanol and then solve it in water.
Hi: I thought you are choosing the right and the common method for extraction the plant materials.
to obtain an plant extract, you must know what kind of compounds are common in the studied plant genus, then you can choose the best method
for example, if you want to extract terpenes, you can extract the plant with chloroform or aceton using extraction with solvent, but if you want the crude extract with the most present compounds in the plant, it is better to use maceration (hydro-alcoholic extraction), or with soxhelt (using methanol or ethanol)
Hi, for extraction you have two type of solvents polar and nonpolar. These type you can use them for extraction any compounds or crud and determinated your compounds
Two types of solvents are there one is polar and another is non polar both you can use for extraction of any compounds even u can do for as aqueous extraction.
After extracting the extract, a set of preliminary tests are carried out to determine the compounds found in the extract. For example, phenol compounds, alkaloids, etc. are detected. After ascertaining the presence of the compound, the compound is extracted with the appropriate solvent and the appropriate medium. For example, to extract the alkaloids, in addition to the organic solvent, The center is acidic and complete the extraction process. We add sodium bicarbonate and so on
the best is to divide the extract into fractions of different polarity, which are easier to analyze, since in total they can be difficult to separate.extract the aqueous extract sequentially with non-polar solvents that do not mix with water, then with chloroform, ethyl acetate, n-butanol. Will remain water part of with polar connections. You will take to study the fraction corresponding to your goals and objectives.
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