Any recommendations for a good (fast, minimal hassle) way of re-prepping an arrayed plasmid library (>1000 vectors) that is running low?
Hi MK,
In what format is your library (96/384 well )? Have you got the vectors in E. coli or are they just plasmid preps ? If in E. coli, surely it's not a problem to find a lab with a colony picker (Q-Pix) with which you can transfer the clones to fresh 96/384 well plates with medium to grow up a fresh batch and prep plasmids from there.
If you have it as plasmid preps, how about (if the plasmids are not too big) doing PCR to amplify the whole vector analogous to when you introduce point mutations (http://www.agilent.com/cs/library/usermanuals/Public/200521.pdf). I know there is a risk of introducing mutations, but if you use something like Phusion polymerase, this should be minimized.
Cheers
Lonnie
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