I am doing a mammalian oocytes maturation, followed by nuclear meiotic evaluation using confocal microscopy, I mount the oocyte over 90% glycerol in a double sided adhesive tape, and I am curious because I don't want to smash the oocytes under the cover slip. is there any recommendation for a good commercial wax cushion to be used in this case. (oocyte diameter is 100 micron).
Thank you,
Yes. It is matter of concern and although alternative methods are available in order to prevent oocyte damage during handling in vitro. The alternative methods have some or other type of negative impact on the oocyte. The development of an improved method/materials that can replace glycerol and prevent damage under experimental condition is required. To the best of my knowledge no such appropriate wax cushion is in the market which can be better in handling oocytes in vitro or for the assessment of maturation process in mammalian oocytes for confocal microscope.
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