Dear Sweilem,

Regarding Neuro2A transfection I would invite you consulting the post in the link below.

In brief, the best recommendation would be the Magnetofection method, with the use of NeuroMag transfection reagent. This reagent was first developped for primary neurons (cortical, hippocampal...) and showed huge efficiency in numerous neural cell types .

for more information, you can have a look at :

http://www.ozbiosciences.com/transfection-cell-specific/52-neuromag-neuron-transfection-magnetofection.html

I'm pretty sure this reagent will give you reliable efficiency, you can contact me directly at [email protected] or via Researchgate if you need more informations or if you want to try Magnetofection.

Good luck for your experiments,

Cedric

https://www.researchgate.net/post/What_transfection_reagent_can_be_used_to_transfect_a_N2a_neuronal_cell_line_with_plasmid_and_siRNA_at_the_same_time

Thank you very much dear Cedric.