Some kits like QIAEX II Gel Extraction Kit are used to obtain bands from DGGE gels, but also some researchers only eluted the fragment in water, how to get the best results?
Sachin A. More
Hi Jazmin,
Your can prefer sambrook for polyacrylamide gel extraction protocol given under dgge chapter. This may help you ......all the best
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Paul V Flanagan
I have only used the water method-it works well as long as you get defined banding in your gel and good separation. I just stabbed the band with a sterile tip, placed it into 50 uL of sterile water and mixed it on a vortex briefly. then leave overnight and use the water as a template for PCR..
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Jazmín Aguilar Medina
Thanks Sachin A. More and Paul V Flanagan, your comments are very usefull
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