I want to Isolate all bacteria from a throat swab and then identify each.
Can you please recommend the best technique to do so.
Thank you in advance.
I just finished writing my review article on rheumatoid arthritis. the review contains about 3600 words (tables and figures included only) and 150 references. The article scored 30% when checked...
08 September 2016 3,259 2 View
I want to do a meta-analysis of the association of a certain gene with RA worldwide.
10 November 2013 8,227 1 View
I have collected about 96 articles concerning rheumatoid arthritis and infectious agents. I will be honored to collaborate with someone experienced in this field.
10 November 2013 3,302 2 View
The antimicrobial effect of local anesthetics has been known for more than 50 years, but it still seems to have an unclear mechanism of action. which bacteria? which concentration? etc etc. ....
27 February 2021 4,450 2 View
Hello I'm currently working on a topic related to studying the anaerobic biodegradation of TPH in marine sediments. In this work, sediment should be mixed with fuel oil to a concentration of 20 g...
24 February 2021 9,839 3 View
Hello everyone, For part of my PhD project, I'm hoping to grow some lactobacillus species in the lab so that I can create standard curves for a qPCR quantification of a faecal sample. However, I...
24 February 2021 3,157 6 View
I will be performing RNA isolation with frozen lung biopsy samples and am in the market for a tissue homogenizer. I am wanting a homogenizer that will fit in a 1.5ml tube. I was looking at the...
24 February 2021 3,623 3 View
Hi, I have been struggling with a weird issue for my RNA samples. I used the Qiagen RNA plus mini kit. For some reason no matter how careful I am with the washes, when I measure the RNA, I get...
22 February 2021 1,286 6 View
I got a thin band and a thick band i guess it would be the genomic DNA and the 260/280 ratio is 3.
21 February 2021 6,523 6 View
I was trying to isolate fibroblastic reticular cells (FRC) and follicular dendritic cells from mice spleen, but kept getting poor yield. I found from literature that the frequency of FDC is around...
17 February 2021 3,506 4 View
I have been reading various articles and most state that treatment with CaCl2 produces higher efficiency of transformation than MgCl2. Can anyone explain why Ca2+ is better than Mg2+ at...
16 February 2021 8,519 5 View
Hello all, I am interested in deleting a highly expressed non-coding RNA located on a Listeria monocytogenes plasmid. Currently, there are not any optimized gene deletion methods for Listeria...
09 February 2021 4,994 1 View
Dear researchers, In literature, so far I am seeing that downdraft is usually used for small-scale purposes (10kW-10MW). One of the reasons behind is "they do not allow for uniform distribution...
08 February 2021 5,403 1 View