If I'm working with Cytokines and Growth Factors whose ranges of stock concentrations are in between1,2,5,10,100 ug/ml...How much Nano grams should I start my trials with Stress Experiments in case of working with 12 removable chamber slides by Ibidi...and adherent cell lines...
- And which should I use to make these dilutions distilled H2O or PBS or with cell culture medium! especially if one of these growth factors will be reconstituted in 10 Mm HCL ?
If you don't have bovin serum albumin as a carrier protein for dilution would it proper to use fetal bovin serum or NGS normal goat serum!
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