I am working with H9C2 cells at the moment, looking at hypertrophic effects of several compounds on the cells. I am using PCR for hypertrophic markers and total protein experiments, but would like to be able to measure cell size. So far I have tried immunostaining for alpha-actinin, but it is just not working in H9C2 cells at all, so it is time to move on to a new method! I have considered using H+E staining, but was told this is tissue specific, and would not work on cultured cells. What would be the best way to stain the cells?
Hi Carla,
I don't know the particularity of the H9C2 cells , but I would like to propose you to try Maygrundwald giemsa . Hopefully it may help with these specific cells
Hi Carla,
This answer may be a little late, but you can use Wheat Germ Agglutinin (WGA). It stains for the glycoproteins on the surface of cell membranes and can stain t-tubules in cardiomyocytes (Savio-Galimberti et al. 2008).
It is never too late kristie.
Thank you for sharing, still helpful!
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