Carla De Villiers

3 Questions 3 Answers 0 Followers

Questions related from Carla De Villiers

What is the best staining technique to measure cell size on H9C2 cells?

I am working with H9C2 cells at the moment, looking at hypertrophic effects of several compounds on the cells. I am using PCR for hypertrophic markers and total protein experiments, but would like...

06 June 2016 4,502 3 View

Can anyone help with low RNA yield with Qiagen RNAeasy micro-assay kit?

I am trying to do an RNA extraction of H9C2 cell cultures - these are rat cardiomyocytes. So far, we have found very low yield of RNA each time (around 1-2ng), despite following the instructions...

06 June 2014 9,307 5 View

Roughly how much RNA do cardiomyocytes contain?

I am working on a PCR of H9C2 rat cardiomyocytes, and was wondering whether anyone had a rough value for the amount of RNA per million cells to help in choosing cell density + plating parameters.

06 June 2014 8,237 0 View