Disperse them in an alcohol,and use a pipette to drop on a carbon film, then vacuuum dry
madam,
Dispersed your compound in ethanol or in distilled water, after that put 30 to 40 minutes for ultrasonication. Add a single drop of nanoparticle solution onto the carbon-coated copper grid (400 mesh). Dry at room temperature. Make sure your grid is not touching the tissue paper. Further, that leave the grid for 30 to 45 min to air dry and then go for imaging.
For more information, please see the following link-
https://www.youtube.com/watch?v=F0ZNUykXovk
Disperse the nanoparticles in ethanol or in distilled water, put the nanoparticles in the sample holder and dry at vacuum. take care that be well disperse and not make aglomerates.
Firstly, check that on which solution Nano particles (which you have synthesised) disperse properly like ethenol, alcohole or water. Prepare a nano particle mixed solution, sonicate it for few minutes. Take a carbon coated grid on a whatman paper, add a 5-10 microleter drop on to a grid using micropipette. Keep it for natural drying. Your sample is ready to record TEM.
I would be careful when using sonication since some nanoparticles may agglomerate in the process instead of being dispersed.
I sonicated my sample still I got the agglomerated particles in the TEM. Can you please explain it?
In TEM sample preparation what quantity of nanoparticle needs to be mixed in solvent ethanol. before ultra-sonication? minimum quantity that would give result.
It depends on the material that you are going to analyze
It's quite easy, just dissolve your sample in water then sonicate it by probe sonicator after that take a small amount of your sample and drop 1-2 drops on the TEM grid and leave it to dry at room temperature.
To get the best result, avoid high concentration.
Dear Dr.
Do all types of materials be dissolved in water? And is there an effect from water on the material characteristics?? @Ali Alsuraifi
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