Has anyone tried to scan fluorescence IHC slides with microarray scanner?

I have an old slide microarray scanner (VIDAR Revolution 4200) and would like to give it a "second life", as IHC scanner array (fluorescence dyes), FISH over tissue or similar. Has anyone tried...

24 February 2021 9,210 1 View

Different antibodies show signal at same MW in SDS-PAGE, what is wrong?

16 February 2021 2,857 9 View

The modern system of international relations: unipolar or multipolar world?

Do you think that there is one center of power in the modern world, or are all countries equal in the international arena?

01 January 2021 7,026 2 View

Mie scattering of a Gaussian with curved wavefront by a sphere?

Can anyone please suggest some references? I would like to simulate scattering of a fundamental mode of a fiber by a microsphere. I am only familiar with a plane wave case, but in case of a curved...

01 January 2021 1,440 3 View

Blood and tissue DNeasy mini kit 260/230 contamination?

I've been trying to extract DNA from rat liver tissue using Blood and tissue DNeasy mini kit. I followed handbook procedure and all of my samples had low 260/230 (

21 December 2020 7,100 3 View

Student's t-test or DeSeq2 for RNA-Seq data analysis?

Hi everybody, I am analysing RNA-Seq data of patient samples. One group of samples has a certain monoallelic deletion, the other group 2 copies of the wildtype gene. Now, I want to compare the...

28 November 2020 5,977 3 View

Cre-loxp system does not work (lines #017455 and #017320, JAX). Why?

We bought this year two lines (#017455 and #017320) to get specific expression of channelrhodopsin2 (ChETA, in this case) in parvalbumin-positive (PV) interneurons. We hoped that crossing a mouse...

16 November 2020 8,701 1 View

Why after cloning recombinant pET in E. coli DH5a I get a very low plasmid yield after extraction?

I chemically transformed a ligation product (pET28a and my gene of 3051 bp) into E coli DH5alpha competent cells. I plated 200 and 800 ml of my transformation on plates of LB agar Kan (25 mg/ml)....

13 September 2020 4,421 3 View

Good markers to distinguish D1R/D2R striatal medium spiny neurons?

Hi! I want to distinguish D1R and D2R subpopulations of medium spiny neurons in striatum in immunofluorescence. Most of the papers I found use transgenic EGFP mice targeting this receptors, but I...

31 August 2020 3,863 2 View

does anyone have the user manual of the geostrain program?

Hello, I am looking for the user manual of the geostrain software, I have downloaded the software but the user manual is not found. I will be very grateful for your help Cheers

14 June 2020 4,169 1 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

Polymer composites suddenly solidifies?

I am making polymer composite by solvent mixing method. When I am trying to add filler solution in polymer solution it quickly get solidified. How to make them then uinform? It doesn't give time...

03 March 2021 7,540 4 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

What solvent can I used for Paal Knorr reaction between 6-amino hexanoic acid and 2.5 hexanedione?

I am try to make the Paal Knorr reaction between 2.5 hexnedione and 6-amino hexanoic acid, my problem is type of solvent to use, because 6-hexaminohexanoic acid is soluble in water but I am not...

02 March 2021 4,443 2 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.

02 March 2021 4,359 3 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View