Elena Venuti

4 Questions 6 Answers 0 Followers

Questions related from Elena Venuti

Buffer or no buffer, and what pH to store enzyme and standard compounds for activity assay?

I want to measure the activity of propionyl-CoA carboxylase (a mitochondrial enzyme found in many tissues). I will be extracting the enzyme from frozen tissue and need to store this somehow....

11 February 2020 2,176 0 View

Is it normal for an intron to not contain any reported SNPs?

I am designing CRISPR Cas 9 guide strands to an intronic sequence for knock-in experiments. I am checking for SNPs/variants in the locations of these guides using the NCBI portal of databases,...

03 January 2020 6,776 3 View

How do I determine the best enzyme model to fit to kinetic data?

I am trying to find the best model to my data. The velocity (V) vs substrate [s] curve looks hyperbolic under normal conditions. When I introduce an inhibitor of interest at concentration of, say,...

31 May 2016 7,598 4 View

What is the best solvent or solvent combination to use when resuspending a film of fatty acid, triglyceride and phospholipid for HPLC injection?

In the literature methanol or methanol : chloroform are mostly used. Is this right?

18 May 2015 8,380 4 View