Elena Venuti

5 Questions 13 Answers 0 Followers

Questions related from Elena Venuti

Buffer or no buffer, and what pH to store enzyme and standard compounds for activity assay?

I want to measure the activity of propionyl-CoA carboxylase (a mitochondrial enzyme found in many tissues). I will be extracting the enzyme from frozen tissue and need to store this somehow....

11 February 2020 2,230 0 View

Is it normal for an intron to not contain any reported SNPs?

I am designing CRISPR Cas 9 guide strands to an intronic sequence for knock-in experiments. I am checking for SNPs/variants in the locations of these guides using the NCBI portal of databases,...

03 January 2020 6,816 3 View

How do I determine the best enzyme model to fit to kinetic data?

I am trying to find the best model to my data. The velocity (V) vs substrate [s] curve looks hyperbolic under normal conditions. When I introduce an inhibitor of interest at concentration of, say,...

31 May 2016 7,656 4 View

What is the best solvent or solvent combination to use when resuspending a film of fatty acid, triglyceride and phospholipid for HPLC injection?

In the literature methanol or methanol : chloroform are mostly used. Is this right?

18 May 2015 8,445 4 View

How do I make a stable and reproducible phospholipid/lipid/bile salt emulsion?

Hi everyone. I am trying to make mixed micelles using purified egg phosphatydilcholine (PC) at various concentrations, glycerol tributyrate and Na+ Taurodeoxycholate, in a pH 8 buffer solution of...

13 October 2014 1,179 14 View