I have tried olive oil as a vehicle but the extract is not also soluble. In fact the extract dissolves in ethanol but crystallizes out on addition of water
You can make a suspension of the ethanol extract in water using Tween-80, CMC or any other suspending agent and administer it orally to the animals for determination of LD50. This is acceptable.
In many cases DMSO is good option. You can dissolve the extract in a small amount of DMSO, and then in a buffer, water, etc.
Try adding ethanol (1% V / V) followed by TWEEN 80 (5% V / V) and top it up with desired amount of water.
Hi the best solvent for meagerment lcd50 for extract that dont solution in water is methanol or ethanol
The extraction can be carried our in alcohal The alcohalic residue is the be dissoved in minimum quantity of acetone and Tween is to be disssoved in minimum quantity of aceone separately. Both acetonic solution are mixxed togather and evaporate acetone to get fluffy soid material. This is soble in water and can be used for LD50 or any other biological/ therapeutic activity.
It depends on the nature of your extract (i.e., its ingredients) and the amount or concentration that will be needed in your research. Different solvents such as DMSO, Ethanol + Tween 80, Ethanol + CMC, etc. can be tried but based on the solvent and its quantity you use to dissolve the desired amount of your extract, you have to employ a control group to receive the same amount of the solvent without extract.
Good Luck
Depends on the nature of its compounds (eg plant extracts or extracts of microorganisms, among others). Here are some examples of good solvents for water-insoluble compounds.
Ethanol 1% Tween 80 at most 5%, methanol 0.5% and the best solvent, DMSO. An important point to note is the stability of the bioactivity of its extracts to different solvents, eg. some substances are unstable in DMSO.
Do not forget to make the control with the same concentration of the diluent.
As Mr. Oliveira and Mr. Jamwal suggestion, we should have take in to account on stability of compound(s)/extract firstly, and how mL you need per dose? (mouse or Rat). Preferably using 1% CMC (in water) than olive oil. Don't forget to make the control, as our friends mention above.
I think 100% DMSO should not be used because of its reaction with the extract. On addition to the extract releases heat to the surrounding. In other words, its reaction with the extract is exothermic.
Ethyl alcohol is found to be useful in extraction of alkaloids from herbs.
- Badges
- Science topic
- Similar topics
- Microbiology
- Assays