Hello Charles.

I've worked with chitosan and we use acetic acid (concentrated) as 1% V/V, in order to dissolve the chitosan. As a initial solution, we use to prepare 10 mg/mL (in water + 1% acetic acid) and then, use this solution to prepare 1 or 2 mg/mL for assays in general.

Hope that can help you

Hi

Thank you. I just clarify my question, is it better to dissolve chitosan in Hcl acid or acetic acid? for in vivo use afterwards. Thanks

Chitosan is dissolved in acidic media such as HCl or HAc, but the molecular weight of chitosan is very important to select one of these solvents and concentrations of them. It's ideal to use HAc (5% v/v) for biological systems. Also, the dissolving of chitosan needs long time vigorous stirring.

Chitosan is dissolved in 2% acetic acid solution.

Chitosan is dissolved in acidic media such as HCl or HAc, If you need to adjust pH by NaOH

Acetic acid is better option but concentration of Chitosan is dependent on MW of chitosan. Which Chitosan are you using (Cs 10, Cs100 etc) is important to fix concentration. PH of solution can be adjusted by NaOH...