How to sterilize a crosslinked chitosan based hydrogel for biomedical use?

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What is the best solvent (and concentration) for biomedical chitosan?

Chitosan will be used for implantable hydrogel device. What is best betwen Hcl or acetic acid for such a final use? what concentration? Thank you very much

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How to check pH of a high strength hydrogel?

We are working on biopolymeric hydrogels. Our system is highly viscous and sticky, and the gel formed are high in strength. We are unable to use pH electrode and pH strip. Please suggest an easy...

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Does anybody know why my chitosan/pva/gelatin hydrogel dissolve in PBS?

My final research is about making chitosan/pva hydrogel for wound healing using gelatin as crosslinker. Last month, i made the thin film hydrogel and it turn well. It was okay. Last week, i make...

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Guidance needed for preparing the hydrogel samples for the XRD instrument?

I'm working with a hydrogel sample and I'd like to perform XRD analysis. Can anyone offer guidance on preparing the hydrogel for the XRD instrument? Specifically, I'm unsure about the best method...

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How i figuer out the weight of curcumin powder?

I am trying to load curcumin emulsion in 4% chitosan gel but i don not know how many mg powder is needed ?

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What are the strategies to improve the stability of chitosan nanoparticles in DMEM (cell culture media)?

Hi, I am synthesising chitosan nanoparticles using the ionic gelation method and I am characterising the nanoparticles in distilled water and in DMEM (cell culture), however the zeta potential in...

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How to load Remedesivir drug into mesoporous nanoparticles?

Hello, I want to load Remedesivir (RNA polymerase inhibitor) into mesoporous nanoparticles (MSNs). The issue is drug is temperature and pH sensitive and it degrades quickly. I need advice on its...

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Any better protocol for entrapment efficiency of curcumin?

I am currently trying to measure the entrapment efficiency of curcumin in liposomes. Typically, this measurement can be done by centrifugation, and I have followed the protocol outlined by Asif et...

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immunofluorescence, False Positive Nuclear Staining in Hydrogel-Embedded Breast Cells?

I have primary breast cells embedded in the hydrogel. When stained with EpCAM without the hydrogel, the staining was correctly localized to the membrane as expected! However, after embedding in...

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Nicotiana benthamiana seeds sterilization methods for tissue culturing?

I have been trying to grow Nicotiana benthamiana via tissue culture. I used ethanol and sodium hypochlorite with different concentrations and time duration for seeds sterilization and lastly...

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Can anyone suggest a method to check whether crosslinking occurred or not?

im working on the photocrosslinking of hydrogels and want to check whether crosslinking happened with the material I have.

12 June 2024 5,579 3 View

Arun Karthick

Try UV and ethanol.

Ramin Javahershenas

Article About the Sterilization of Chitosan Hydrogel Nanoparticles
https://doi.org/10.1002/1097-4636(2001)58:1

Charles Calle

The hydrogel is a copolymere actually, contaning amide too with chitosan, so possible maillard reaction. Looking for tips or publications to sterilize the crosslinked hydrogel at final step stage, just before tissue engineering use. Thanks a lot