If possible, you can purify the IgG antibody of goat and cattle, and prepare the secondary antibodies. But this will cost a longer time.

Thanks Yang Hong for the excellent suggestion. We shall hope to achieve this in the future as we currently lack capacity to perform antibody purification in our lab. Meanwhile I was thinking the commercially available rabbit anti-goat IgG could be used against goat and cattle serum/urine.

I think any of the polyclonal antibodies for rabbits would do. Detection of antigens rather than antibodies, on the other hand, appears to be a suitable approach in the diagnosis of HF. Please See the attached

Thanks @ Victor Emojevwe your suggestion and the material is appreciated.

Regards