Hi,
I have been looking into best Reference gene in Saccahromyces cerevisae (yeast) for Gene expression study RT-PCR especially to study along side URA3 gene as my gene of interest however literature have several. please input your thoughts on it?
Hi Fg!
See this paper.
Article Validation of reference genes for quantitative expression an...
I would say ALG1 is the most reliable.
I’ve always used GADPH or ACT1, interesting to read the paper cited above warning against ACT1.
Hi! It depends on the condition, actually. ACT1 seems to vary depending the condition (and maybe the strain that you are using). I used it because I had RNAseq data that showed it was good (I checked it by RT-PCR too). I am working with S. pastorianus.
I saw in one paper that ALG9 was a good candidate, but again, you should check if it works for the conditions that you want to test.
Good luck! 😁
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