What is the best protocol for erythrocytes (RBCs) isolation from blood (300mL)..?

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Can anyone suggest papers in the hybrid auto-mata area that models cyber-physical systems in power systems?

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What is the recommended age limit for primary posterior capsulotomy in pediatric cataract surgery?

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27 December 2020 4,268 6 View

Increase coercivity with device size (multi-domain) ?

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Software for L x T mating design analysis and heterosis estimation?

Kindly suggest the name of free softwares available for LxT analysis and heterosis estimation in crop plants.

22 October 2020 1,744 10 View

I have performed XRD on Fusion zone of 316L stainless steel as well as incoloy 800. Why only one or two peaks are showing?

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Please suggest the name of important and free softwares for attractive NJ tree, dendrogram and PCA diagram?

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Band decomposed charge density using quantum espresso ?

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How to find out the Compund Average Annual Publications Growth Rate (CAGR) in Bibliometrics ?

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How the INCAR file is different for SCAN and PBE+U for VASP?

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MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

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MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

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fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

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fatal error

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Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

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What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

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Is there any test to determine the cardiovascular risk of patients with obesity? from the perspective of obesity?

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How to repeat an operation on an atom selection for multiple times in PyMol?

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Rosemary L Sparrow

Hi Indrajit

Some questions.

Is the blood freshly collected?

How was it collected?

Is it human blood?

Regards, Rosemary

Indrajit Sahu

Hi Rosemary L Sparrow,

Thanks for your concern.

Yes this is Human blood and collected from blood bank (approx 1 month old).

Thanks.

Gregory Barshtein

Hi Indrajit,

It is well documented that long storage of RBCs cause to hemolysis.

I recommend to decrease centrifugation speed (we used 500 xg) and to verify PBS composition.

Regards

Hi again Indrajit

There will be a lot of damaged and fragile cells in 1-month old blood and repeated washing will cause further damage, as you have found. Can you obtain fresher blood from the blood bank?