3 Questions 12 Answers 0 Followers
Questions related from Indrajit Sahu
I am trying to prepare cell lysate from mice skin epidermal tissue. I separated epidermis from dermis by 1X trypsin. Then reconstituted with RIPA buf, then homogenize, sonicate and centrifuge. But...
07 February 2017 2,566 4 View
Hi All, Can anyone suggest any methodological detail to quickly separate haemoglobin from my erythrocyte cell lysate..? (And most importantly I have to use detergent free Lysis buffer for my...
02 August 2016 1,695 4 View
I am trying to isolate RBCs from blood, nearly 300mL. Can anyone suggest the best protocol for it? I am following slow-speed centrigugation method with washing the cells with cold PBS. Overtime I...
02 August 2016 340 4 View