Indrajit Sahu

3 Questions 13 Answers 0 Followers

Questions related from Indrajit Sahu

What is the best method to prepare cell lysate from mice skin epidermal tissue?

I am trying to prepare cell lysate from mice skin epidermal tissue. I separated epidermis from dermis by 1X trypsin. Then reconstituted with RIPA buf, then homogenize, sonicate and centrifuge. But...

07 February 2017 2,640 4 View

How to separate the huge amount of Haemoglobin from the erythrocyte cell lysate?

Hi All, Can anyone suggest any methodological detail to quickly separate haemoglobin from my erythrocyte cell lysate..? (And most importantly I have to use detergent free Lysis buffer for my...

02 August 2016 1,741 4 View

What is the best protocol for erythrocytes (RBCs) isolation from blood (300mL)..?

I am trying to isolate RBCs from blood, nearly 300mL. Can anyone suggest the best protocol for it? I am following slow-speed centrigugation method with washing the cells with cold PBS. Overtime I...

02 August 2016 396 4 View