I study fish telencephalon both by electrophysiology, by localization of receptor and ion channel expression and anatomical connectivity to better understand how this "small" neuronal network functions.

I would like to establish the neural connectome of the telencephalon, at first at a gross resolution, but on the long run at the fine scale. what are the key features I have to think about and include in my dataset?

What would be the best way of doing this? What would be the best software, freeware, what-ever-ware to use, both for collecting the features of the nodes and edges, and later for analysis?

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