Hello! I have been having issues successfully performing media changes on cells/groups of cells that are in Kelco Gel (KG). The function of KG is to keep cells in suspension so as to form a 3D suspension based media. However, this means that it is very difficult to get the cells out when performing a media change, even when following the dilution, centrifugation speed, and procedure listed in protocols I've found, I'm still losing cells with each media change. Does anyone have experience with this material and how to get the cells to separate out from the KG without being damaged?
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