What is the best primer of choice for real time RT-PCR?

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How to increase the transcription efficiency in HEK293 cells using PEI?

Get more than 80% - 90% of transcription efficiency

19 July 2023 7,912 3 View

Is it possible to use In-Circuit Emulation of ARM processor to replicate the Matlab model. Is there any software to put the code into ARM chip?

Is it possible to use In-Circuit Emulation of ARM processor to replicate the Matlab model. Is there any software to put the code into ARM chip? Please suggest on this.

25 April 2023 9,820 2 View

I Want a plagiarism software for my personal use , please share where it available (Link) , price detais?

My own question

15 March 2023 6,905 7 View

What is the difference between feature influence vs feature effect in machine learning (for land use science)?

I’m an ecologist using machine learning algorithms (using the gradient boosting approach for the first time 😊) to gain insight into land use patterns. My question is related to a machine learning...

22 January 2023 5,967 11 View

Is there simple and efficient way or any parametrization method to estimate glacier basal velocity for a valley glacier?

Being an essential parameter, basal velocity estimation of a valley glacier is still not significantly discussed which helps in assessing the glacier water storage capacity over a period and...

02 November 2022 8,153 0 View

Poly Ethylene Glycol 6000 Measurement?

How to measure/convert the Mpa (PEG-6000) to Grams? for example -2.0Mpa = how many grams?

30 October 2022 8,285 3 View

Why the mass conservation ice thickness model approach is superior to the other ice thickness model approaches like GlabTop or velocity-slope?

Most of the studies related to the High mountain glaciers adopt a mass conservation approach at a global level while other approaches are least adopted.

06 February 2022 9,985 0 View

Adhesive for contact lenses ?

Hi Everyone, We need to attach metal beads to contact lenses for a project, and I could not find any adhesive that works with contact lenses (preferably biocompatible) . I know that they are...

01 February 2022 1,887 2 View

In gabbro rock LREE shows enriched pattern pls explain about magma emplacement and tectonic setting of this rock ?

n gabbro rock LREE shows enriched pattern pls explain about magma emplacement and tectonic setting of this roc

13 December 2021 8,321 5 View

Where to get permission to use the Occupational Stress Index in my dissertation?

where to get permission to use the Occupational Stress Index in my dissertation? Can i know about the interpretation?

26 September 2021 6,700 3 View

How to confirm the site-directed mutagenesis result without performing NGS?

I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...

08 August 2024 4,645 2 View

I can't see the ssDNA band after performing asymmetric PCR. Is there any way to do this?

After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...

08 August 2024 1,668 3 View

Research Methodology - Impact of Corporate Reputation on Stakeholders Behaviors?

Please can anyone support with the survey questions based on RQ measures and propose how to do it in FMCG industry and include as well the role of brand equity Thanks

06 August 2024 949 0 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

05 August 2024 8,835 1 View

Why after performing site directed mutagenesis ,I don't see any colony after transformation?

I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...

05 August 2024 9,059 3 View

Anyone having idea about VN primer for miRNA primer design ?

How to design VN primer to attach with universal reverse primer

05 August 2024 2,116 3 View

Should I remove an item from a scale to raise Cronbach's alpha and McDonald's omega or is it better to leave it if they are both over .7 already?

Hello! I have this scale which had 10 items initially. I had to remove items 8 and 10 because they correlated negatively with the scale, and then I removed item 9 because Cronbach's alpha and...

01 August 2024 4,606 7 View

Does anyone have issues using Prepman Ultra reagent for MicroSeq ID bacterial, fungal and yeast sample preparation?

I have been attempting to extract DNA from Bacterial, Fungal and Yeast banked samples (>1e7 cells) using Prepman Ultra reagent and I seem to be struggling to obtain a sequence. Although the...

01 August 2024 2,079 0 View

I'm optimizing a tetra-ARMS PCR protocol with amplicon sizes: 165bp, 123bp and 93bp. Why, in the gel, only 165bp is visible while I'm expecting all 3?

It's an end-point PCR protocol. I'm using 1.5% agarose gel with SyBR Safe dye and TBE as a running buffer, visualization on BioRad XR+ system. I was primarily thinking of primer efficiency,...

01 August 2024 4,673 4 View

PCR showing no bands - Master mix and primers didn't mix?

Hello everyone, I performed a PCR yesterday, and the results showed no bands on the gel. Of course, I probably missed some crucial steps, like adding my samples to the PCR strips themselves, for...

31 July 2024 2,406 6 View