What is the best or most accepted approach to quantify immunoreactivity for androgen receptor by immunohistochemical staining using light microscopy?

More Nisreen abu shahin's questions See All

Methodological confusions regarding deductive and inductive reasoning, hypothesis and theory test?

Hi Colleagues- What do you think about the following methodological issues: 1. Is it methodologically wrong to formulate quantitative research questionnaire based on inductive reasoning? 2. Is it...

21 February 2021 4,634 6 View

Are there alternatives to the Devarda alloy when estimating nitrogen?

Are there alternatives to the Devarda alloy when estimating nitrogen? devarda alloy is reducing the nitrate to ammonium

15 February 2021 8,838 3 View

Up to what extent website personalization can help in creating urge to buy impulsively?

Up to what extent website personalization can help in creating urge to buy impulsively? The digital transformation is making most of the organizations to go online and start doing business through...

09 February 2021 4,430 6 View

Observation instrument for classroom activity in consecutive interpreting?

Hello everybody My research involves an intervention where an instructor delivers a training course to students. My role as a researcher is to write down observations. Do you have any idea about...

03 February 2021 6,853 3 View

Can anyone help to convert ODK XLS into XML?

We want to conduct a verbal autopsy using WHO adult VA updated tools in ODK. The current version of WHO adult verbal autopsy tools is v1.5.3. I have successfully converted VA ODK-XLS v1.4 into XML...

03 February 2021 844 2 View

What is the best way to encode categorical variables in datasets with many features and avoid increasing dimensionality of the dataset?

I am working with a large dataset that has many categorical variables with many classes in each variable. I want to encode the categorical variables without increasing the dimensionality of the...

31 January 2021 6,986 3 View

May I know how to determine the acceptable intakes for new types of nitrosamine discovered in drug?

Currently only common nitrosaminse like NDMA and NDEA have mentioned the acceptable daily intake. Based on EMA guidance, should refer to ICH M7 chapter 7.5 guideline. I have read but could not...

20 January 2021 522 1 View

How do i analyze scratch assay data obtained thru imageJ?

hello. i manually measured the area in each image. the output i got was area/mean/min/max then i put all the data into excel, and also added the following columns: group names/date/hour pic was...

19 January 2021 3,936 3 View

What are the alternative to Rotary Evaporation?

I want to remove solvent to obtain a solid . i would like to remove solvents such as THF, TEA, carbon tetrachloride . the obtained solid which is a Phosphorus ester. What could be an suitable...

04 January 2021 1,220 1 View

ENVI error when opening Metadata?

ENVI software (V 5.3) has a problem with opening some Metadata files. An error appears which means it does not seem to be a Metadata file. How can I solve this problem?

20 December 2020 4,590 3 View

How do I determine the degree of polymerization of PEG 400 based on NMR integration?

Hello, I am working on the photocatalytic degradation of PEG 400. I would like to know if it's possible to determine the degree on polymerization based on NMR integration, or by the MW of the...

02 March 2021 6,186 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

Anyone know of a good progesterone antibody?

I am looking for a good progesterone antibody to quantify levels of progesterone in zebrafish. Applications would be ELISA, and immunohistochemistry.

28 February 2021 5,929 2 View

How to include original NMR data in a manuscript?

hello. i am junior researcher. I received a comment from a reviewer that he could not find NMR data in my submitted paper. I had included the NMR graph with explanation and formula to calculate...

27 February 2021 734 4 View

B-mercaptoethanol (BME) protocol?

Hi everybody, I have been running a multiplex IHC which allow me to stain the same tissue with different markers (Primary Ab + Secondary conjugated Ab) (AF487, AF555, AF647). Since I would like to...

27 February 2021 5,028 2 View

Why do my MCFs not form monolayer?

Hello, I am looking for some expertise on culturing MCF7s. I purchased them from ATCC and I have been following all media and culture conditions. Media is EMEM with 10% FBS and 10ug/mL of...

27 February 2021 3,904 2 View

Is there anyone who experienced with cytotoxic effect of Hoescht 33342 dye?

Is Hoechst toxic to cells? In general, it is known as non-toxic. I am working with dsRED MDA-MB-231 cell line. I applied it for my PDMS-based microfluidic platform, for this reason, I incubated...

26 February 2021 9,119 3 View

Why my agarose gel staining sometimes looks not homogenic but like gradient (see the figure)?

I am worried about this overexposure of the upper part of the gel in the picture. Is it possible that this is the result of too much concentration of ethidium bromide? Why is there such a big...

25 February 2021 8,140 3 View

How do I transfect a large plasmid into PC12 cells with nerve growth factor (NGF) receptor?

I have a large 16 kb plasmid, which I need to transfect into PC12 cells. Lipofectamin 2000 didn't work and with GFP alone the transfection rate is very low. I also tried the neon invitrogen...

25 February 2021 6,635 5 View