Selecting particular columns from large datasets (csv) using R Studio?

So I have large number of csv files (100+) in a directory with same structure and column names. The data is in time series and there are approx 30 columns/file. However i only want to aggregate...

08 February 2021 245 5 View

How can solve the second order linear homogeneous partial differential equation with variable coefficients?

can someone help me with reduced the partial differential equation du/dt = - f(x) d^2 u/ dx^2 - g(x) du/dx --- (1) to become: du/dt = - p(x) d^2 u/ dx^2...

21 January 2021 3,296 1 View

IHC-paraffin experiment: how do you approach shopping for an antibody?

Hello everyone! I am shopping for an arginase 1 antibody to use for IHC-paraffin experiments with rat tissue. So far what I have understood is that if an antibody says it is reactive with rat,...

16 January 2021 794 3 View

How to explain insignificant independent variable which is significant with interacting (Moderation) variable?

10 December 2020 3,037 1 View

What could be the impediments to the initiatives adopted by the bank towards FI in the Indian scenario?

this question from case Union Bank of India: Initiatives towards IT-enabled Financial Inclusion

09 December 2020 7,721 3 View

Can we use the general hotplate as a source of heating in the Soxhelt method or not ? what is optimal temperature when using n-hexane as a solvent?

there is a problem with the heating part in soxhelt apparatus, so my question can we use the standard hot plate as a source of heating?

05 December 2020 9,322 2 View

Carbon Emission calculation factors ?

I am thinking about writing a paper on carbon emissions based on electricity consumption data from some buildings. From a research perspective, which factors should i specially focus while...

09 October 2020 4,142 3 View

How to calculate the quantity of enzyme needed to hydrolyze protein?

I have alcalase enzyme it's activity 10000U/g and I want to use it in hydrolysis whey protein isolate 90% protein. A suspension of 5% (w/v)WPI prepared so I am asking about the quantity of enzyme...

05 October 2020 9,825 3 View

Will the publishing of conference papers be counted?

During promotion, papers published as conference proceedings are not considered; on the other hand, it is counted as publications in a few places but with less credits. kindly request your...

23 September 2020 3,254 38 View

Which configuration should be more preferred AC or DC coupled for PV-battery storage systems?

The ideal assumption is : uniform sized PV and battery storage system, and similar load for both configurations.

07 September 2020 7,193 30 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Does any one know how i can prepare liver tissue with transmission electron microscope with good fixation?

i have problem in preparation of liver tissue with bad cell organelles and how i can adjust osmolarity

03 March 2021 8,890 1 View

Chlorinated organic formation in odor control scrubbers using hypochlorite?

I'm involved in a study of odor control technologies for municipal wastewater treatment plant. One of the control options involves a chemical 2-stage (acid/alkaline) packed bed scrubber. The...

03 March 2021 3,661 2 View

What solvent can I used for Paal Knorr reaction between 6-amino hexanoic acid and 2.5 hexanedione?

I am try to make the Paal Knorr reaction between 2.5 hexnedione and 6-amino hexanoic acid, my problem is type of solvent to use, because 6-hexaminohexanoic acid is soluble in water but I am not...

02 March 2021 4,443 2 View

Ester cleavage conditions?

Hi all, I need to cleave the ester in Ethyl 2-amino-1H-imidazole-5-carboxylate to make the carboxylic acid and retrieve the product from solution to use in downstream applications of linking to...

01 March 2021 8,766 3 View

How to remove DCU and HOBT from my reaction?

I am doing a acid and a chiral amino alcohol reaction using DCC and HOBT in DCM or THF? Can anyone suggest me how to remove DCU and HOBT from a reaction mixture??

28 February 2021 2,153 1 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

What is the most common number of epitopes in an multi-epitope Vaccine?

hi everyone, I'm interested in the field of vaccine design specifically multi-epitope cancer vaccines , I want to know if there is a minimum and maximum number of epitopes we could use in a...

27 February 2021 3,882 3 View

B-mercaptoethanol (BME) protocol?

Hi everybody, I have been running a multiplex IHC which allow me to stain the same tissue with different markers (Primary Ab + Secondary conjugated Ab) (AF487, AF555, AF647). Since I would like to...

27 February 2021 5,028 2 View