What is the best natural acidic stains that can be used in tissue staining (except eosin and chromotrope) ?
Curcuminoids make excellent acidic stains. They are easily extracted from turmeric with 70% ethanol. I usually do a 10-15% (w/v) solution. Spin it down, pour off, and filter the supernatant.
How can I define Planetary motion in ABAQUS CAE, how define motion of three rollers around a central axis and passive rotation about their own axis?
16 July 2024 4,145 1 View
Hi to all of you! My goal is to create synthetic silica beads. These beads ought to have a specified size. Could someone please advise on how to proceed or give me the articles I need to finish my...
02 July 2024 8,170 1 View
Hello, I am having a difficult time finding the incident plane wave wavelength dependent spectra of the lights source to find the EQE. Please advise.
26 June 2024 7,274 1 View
Hello I am seeking detailed protocols for the molecular diagnosis of Friedreich Ataxia. Best wishes Saqib
13 May 2024 1,448 2 View
which is the most successful and guaranteed method, cause I Tried sodium fluoride 20mg/kg and it didn't work
08 April 2024 8,414 0 View
IDF curves usually developed using the sub-daily rainfall data. If I use daily data and construct 2,3-day IDF curve, then what are the practical applications of such long duration curves? Thank you.
28 March 2024 918 0 View
I need an expert to help me in assessing the environmental impact of Municipal Solid Waste Management using one of the following programs: SimaPro. ,GaBi. ,OpenLCA. ,Wrate or any suitable software...
20 February 2024 7,755 2 View
Hello dear colleagues, what kind of research I can conduct on Aspergillus fumigatus isolates apart from genotyping?
05 February 2024 3,352 0 View
Hi, experts. I'm trying to expressed recombinant transmembrane protein incorporated with nucleophilic non-canonical amino acids(ncAAs) by genetic code expansion (GCE) in E.coli. I replace tyrosine...
03 February 2024 2,403 0 View
With climate change, high temperatures, and scarcity of rain in Iraq, we find it difficult to determine future agricultural plans based on the abundance of water and temperatures and providing...
25 January 2024 7,412 3 View
I am staining some brain sections stored in cryoprotectant that express a Histone H2B- GFP fusion protein that were generated ~10 years ago. I know I need to enhance signal with an anti-GFP...
07 August 2024 5,338 2 View
Hello What should be done to separate and identify organic acids in HPC when their RetTime is the same?Like oxalic acid with Propanoic Acid.or acids that have a very close RetTime.
07 August 2024 8,782 3 View
Hello everyone, I have recently started using the microtome device for sectioning of paraffin-embedded mice lung. While I had some success in sectioning and observing proper ribbons, some of my...
06 August 2024 666 4 View
To compare positive and negative cell populations in flow cytometry, should I compare unstained cells with antibody stained cells? Or with the isotype control? Most papers show comparison with...
06 August 2024 6,728 6 View
Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...
06 August 2024 9,710 1 View
Dear readers, Thanks for your attention. I am wondering about the health economic problem of quantifying the value of interventions which a) prevent, b) improve symptom profile and c) ultimately...
05 August 2024 3,246 1 View
I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...
04 August 2024 501 2 View
I heard an explanation about something being a better proton acceptor or lone pair donor but that doesn't make sense. I couldn't explain in in terms of acid-base theory. The hand-waving way I saw...
03 August 2024 9,918 0 View
Hi guys If anyone is currently working on aging cells, you guys would like to give me some advice. I'm testing against biomarker (SA-beta-Gal), I encountered a false positive in the control group...
02 August 2024 6,735 1 View
All plants are green but some of these plants becomes yellow. I did not found any reason. Please help me to find out the real problem.
01 August 2024 589 4 View