-using what buffer
-the protocol must be withe a reference (used befor in a scientific research )
This is a very vague question, give the details of your sample.
Method can be chosen depend upon the samples. there is four methods are Extraction, Precipitation, ultracentrifugation, and Chromatographic.
Please look at the following below links which may help you in your analysis:
http://wolfson.huji.ac.il/purification/PDF/Protein_Expression_Extraction/GE_ProteinSamplePreparationHandbook.pdf
https://file.scirp.org/pdf/ABB_2013043017032856.pdf
http://www.101bio.com/files/manual_P502.pdf
https://www.mobitec.com/cms/products/bio/03_proteomics/total_protein_extraction_for_animal_cultured_cells_and_tissues.html?pdf=SD-001_SN-002.pdf
Thanks
10 November 2018 6,016 1 View
proteines extraction and identification
10 November 2018 4,179 3 View
Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...
03 March 2021 2,037 3 View
Hello! I want to quantify by ELISA the secreted (from platelets poor plasma) and the non-secreted (from platelet lysate) PF4 before and after TRAP stimulation. I will use the ELISA from R&D...
03 March 2021 1,499 2 View
Hello, If i am doing a buffer exchange for an antibody of 1mg/mL, does the elution lose protein in the process of buffer exchange? For example, if i flow through 500 uL of 1mg/mL sample, and...
03 March 2021 6,299 3 View
dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...
03 March 2021 6,994 5 View
Hello all, I'm currently working on my undergraduate thesis and I'm having difficulties trying to access KLD Stats and Sustainalytics because my campus Airlangga University and apparently other...
02 March 2021 8,525 1 View
Hi everyone, I'm studying Marketing and I would like to write my PhD thesis on the topic of pricing. Any specific ideas?
02 March 2021 9,706 5 View
02 March 2021 3,060 3 View
02 March 2021 932 1 View
how to calculate BEHAVIOURAL REGULATION IN EXERCISE QUESTIONNAIRE BREQ 3 ,which contain 24 questions and each question has 5 answers (0- Strongly disagree , 1-disagree, 2- Neither agree nor...
02 March 2021 811 3 View
Please provide Book Title and author name
02 March 2021 9,059 3 View