Greetings,
Sterilize the film then cover it with a very thin layer of Muller hinton agar. inoculate your test microbe with a known number of cells and incubate. Count the growing colonies and compare it with the control. Good luck.
Regards.
Greeting,
You can add film (1*1 cm) to Muller hinton broth and inoculate with bacteria test after 24 hours than with tube count Muller hinton broth with bacteria only (Control) .
The inhibition percentage =( Control - test/ Control)* 100
I would use an adaption of BSEN 13697 using the film as the test surface or use the impregnated plastics test - i cant remember the exact name but it should do what you are after.
You can use different techniques to test antimicrobial activity of biopolymeric food packaging films. Please see the attached literatures
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