Various studies have used DMSO as a solvent but it is not dissolved. Some others have used autoclaved water. When we pore that suspension to the media plate it will be settled down at the bottom during the incubation period.
A solution that should work would be to grow the microbes in a broth and put them on a orbital plate to mix the contents with the sample.
I am trying to find the fraction metabolized of a drug by CYP and UGT enzymes. I am unable to get the values of fraction metabolized (Fm) directly from the literature. Can we calculate the Fm of a...
21 February 2021 5,379 2 View
I am trying to use a UDF for velocity input in the room from a fan. I have created a UDF but when I am trying to load the UDF it shows an error stating that it is not compiled for parallel...
03 February 2021 2,187 3 View
Hi all, I just want to know that, if we keep the siRNA in a -20 degree freezer (as mentioned in the product detail), what will be the average life of the siRNA? Can we store for more than one...
10 January 2021 6,346 6 View
This is to design a protocol in which color obtained on nitrocellulose membrane can be converted or processed through any method to digits (numbers) to compare with different state of particular...
06 January 2021 9,309 1 View
Almost all the literature shows that the vertical distributions of soil organic carbon (SOC) & available nitrogen (N) is found decreasing with increased value of bulk density with respect to...
21 December 2020 670 10 View
I want to know if difference-in-difference approach can be used for two treatment groups. Can I use it it between them or should I first identify a control group and then compute the diff-in-diff...
20 December 2020 2,825 1 View
Sediment is relatively younger than the soil in the depositional environment as the sediments are consequence of the accretion of particles transported either by waters or by winds, whereas, soil...
12 December 2020 4,822 18 View
Bulk density of a forest soil sample in a community forest created under social forestry scheme in the district of Nadia, West Bengal is obtained as 0.81 gm/ cubic centimetre, comparatively too...
30 November 2020 4,706 33 View
Hello everyone, I am working in breast cancer and wanted to do in vivo studies in breast cancer mice models. Here we do not have the facility to maintain immune-deficient mice, and I have MCF-7,...
27 November 2020 1,638 3 View
The content of carbon in the biomass varies from 35% - 65% in one literature, in the another, it's 45-50%, though 50% is often taken as default value, and all they are surprisingly constant...
23 November 2020 1,736 7 View
When I conducted the degassing experiment using decompression under anaerobic condition(and I used ferrous based chelating agent), the oxidized ferric were observerd. So, could you tell me the...
03 March 2021 4,892 5 View
We have HUVEC bought from Invitrogen. Cat no -C-003-5c. while first plating the cells we have bought the protocol suggests not to centrifuge the cell with media to remove the DMSO. Should we...
02 March 2021 3,713 2 View
We have synthesized various metal complexes with different environment around the metals. Now to see the application of synthesized complexes we need this study also. we are also interested in...
01 March 2021 1,899 2 View
[Synthesizing Cu nanoparticles without using chemical reductants]
01 March 2021 8,359 1 View
How would define the "good" and "poor" distribution of particles within the matrix. Is there a quantitative solution to assess the distribution of the particles, which then having denotation of...
28 February 2021 7,397 8 View
After I cryofreeze the cell do I need to remove the DMSO for culturing the cell for next passages .
28 February 2021 7,738 3 View
I want to use 0.1% gelatin. Should that be made in PBS or sterile water? Should I autoclave the gelatin soln after making it in water/PBS?
28 February 2021 274 2 View
What could be the reason of moisture at the sides of Vial when I am preparing my polmyer+DMSO solution for membrane fabrication? What etiquettes I am possibly missing here?
28 February 2021 6,516 5 View
Is the period to autoclave not enough? The inoculation in a hood and flame and UV and alcohol.
27 February 2021 9,356 3 View
I would like to synthesize a transition metal doped TiO2 via solgel method. Hence, I would like to know which metal salt precursor to choose among Chloride, Nitrate, and Sulphate. A clear...
27 February 2021 4,285 3 View