Up to my knowledge the following paper is good for Paramecium culturing:

Mitchell, S. A. "A technique for the intensive culture of Paramecium (group caudatum Parameciidae) as a first food for ornamental fish fry."Aquaculture 95.1 (1991): 189-192.

Good luck with your project

thank you very much for your answers

Method for cultivating

Using three glass bottles of about 2 liters with aeration. Fill bottle with tap water and 15 grams of fresh crushed leaves of lettuce. After 24 hours of rest are added paramecium, with aeration placing them near the light. After four days at a temperature of 24-26 degrees Celsius, lettuce this mostly dissolved and observed against the light clouds of thousands of Paramecium. These should be used in the next two days, because after this time the culture reaches its peak population growth, and this brings the appearance of limiting factors that cause population decline.

There is a simpler method, but with lower production. In an about 4 liter flask placing an aerator for the water to move not produce odor. Added a little bit of milk powder (+/- 60 mg per 100 ml water) and some fish food flakes. Placing them near the light source, inoculate the paramecium. Finally, there is another method. To deposit in the aquarium

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"A floating green plant stems and leaves as thin as blades. It has the virtue of creating the infusoria and serves as a refuge for young fish. Vote some paramecium near it and add a few drops of milk once in a while. The paramecium grow and multiply will be between plant.

It depends on how many cells you want to obtain. If the pupose of cultivation is immunocytochemistry or in situ hybridisation the best thing is to cultivate using the following medium. Boil lettuce to obtain yellow liquid (it must not be too dark), filter it trough a filter paper and boil again.  Bring to room temberature and inoculate with Enterobacter aerogenes. Incubate overnight at 30C or at room temperature. Put paramecia in tubes and add the medium. Keep the tubes either at room temperature or in the range of 16-25C.I cultivate my ciliates adding medium every second or third day. If the cells are not in the upper level of liquid, discard the upper level and add the medium. When the  tube is filled either make two tubes or transfer the cells with the upper layer to the new tube. Transferring cells to the new tube is essential if you want a good clean culture, but it is not easy to get many cells (for electrophoresis) with this method.  The other way is cultivating cells in a 1-3-liter flasks in the same medium, doubling the volume of medium everysecond day. To transfer the cells collect them by putting in the narrow-necked flask and adding fresh medium up to the top. The next morning the cells should be in the narrow part of the flask. To maintain them further just transfer to the clean flask. But do not keep the too long after having concentrated them. Good luck!