For DMSO analysis using Headspace Gas Chromatography (HS-GC):

**Parameters:**

- **Headspace:** 40-80°C, 15-30 mins equilibration, 0.2-2 mins pressurization

- **GC:** Nonpolar column (e.g., DB-5), 40-60°C initial, 150-250°C ramp, 200-250°C injection

- **Solvents:** Water (minimal interference), Organic solvents (e.g., methanol, acetonitrile, dichloromethane)

Validate the method for accuracy and precision in your specific application.

Thank you Dear Dr Balamurugan