I am working on endophytes in cotton. I need to isolate endophytic fungi and bacteria from leaves, stems and roots. Plz suggest a protocol where we can easily exclude saprophytes and isolate only the endophytes.
surface sterilisation is the best way to eliminate saprophytes and "epiphytic" organisms from the plant part who's the object of isolation. We should make under consideration the hsitsological (specific tissues) and anatomical nature of the plant part.
Most scientific works on endophytic fungi and bacteria of crop plants was focused in roots, mainly for agrotechnical purposes.
For papers, you can read these:
You can also have valuable technical advice from Prof. Pirttilä, Anna Maria
Thank you very much Oussama Ali Bensaci. I have followed the method as mentioned in Li et al., 2014. The papers you have attached are really very helpful and hopefully I will contact Prof. Pirttilä, Anna Maria for further information. Thanks again...
Hope this information can be useful for you,
J Hallmann, G Berg, B Schulz 2006. Microbial root endophytes. In: Isolation procedures for endophytic microorganisms. Springer.
Best regrads,
Luis
Thank you very much Dr. Luis. The source you recommended is highly useful for further studies. I am grateful to you.
American Journal of Plant Sciences
Vol.06 No.19(2015), Article ID:61938,15 pages
10.4236/ajps.2015.619313
Isolation and Identification of Fungal Endophytes from Grasses along the Oregon Coast
Ruth C. Martin*, James E. Dombrowski
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