Dear Shridevi,

thank you for sharing this interesting technical question with other RG members. DCC is a standard reagent for amino acid coupling. The abbreviation DCC stands for N,N'-dicyclohexylcarbodiimide, Cy–N=C=N–Cy (Cy = cyclohexyl). In general, carbodiimides, R–N=C=N–R, can be regarded as the nitrogen analogs of carbon dioxide, O=C=O. The main principle of the amino acid coupling is based on the fact that the carbodiimides can capture one molecule of water to form the corresponding disubstituted urea derivatives, R-NH-C(=O)-NH-R. There is a vast body of chemical literature about this method available. I suggest that you first search the "Publications" section of RG for papers which have been posted as public full texts.

For some initial information abut the amino acid coupling using DCC you might want to watch the following instructive tutorial video:

Preparation of amides using DCC

https://www.khanacademy.org/science/organic-chemistry/carboxylic-acids-derivatives/reactions-carboxylic-jay/v/preparation-of-amides-using-dcc

Good luck with your work and best wishes, Frank Edelmann

Thank you so much sir.

Dear all, always for controlled peptide synthesis, protection chemistry is a must, the most famous strategy in doing that is the Merrifield approch (Pioneered by Robert Bruce Merrifield), knowen as solid-phase peptide synthesis (SPPS). Once this is respected, then activators come after. My Regards