What is the best method to determine the immune response after PLGA injections in mouse fad pads?

More Sarah Berndt's questions See All

What is the maximal PLGA concentration for fat injections purposes?

Hi Colleagues, Any experience with PLGA injections into mice? What is the maximal concentration I can inject without blocking a 22G needle? I often see data with people injecting 10 mg, can I go...

06 July 2015 4,059 3 View

How can I detect differences in P53 acetylation in HUVEC by western blot?

Dear Colleagues, we would like to evidence differences in acetylation of p53 in HUVEC cells by western blot or flow cytometry, could you provide us with a good protocol? Thank you very much

01 February 2015 5,886 4 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View

What analysis to choose with a large neste dataset and clearly skewed or kurtotic distributions?

Hello! I have a dataset of n=3000 nested within 8 countries with approximately 200 or 400 responses in each country. I originally planned to perform multilevel modelling with 4 dependent variables...

02 March 2021 6,865 1 View

IHC on mouse liver hepatocytes?

Can anyone help me choose primary antibodies for mouse liver hepatocytes and also in conjunction Cd8 cells. Also Cd3 cells. I want to look at hepatocytes and adjacent immune cells in mice with...

01 March 2021 6,953 1 View

How to avoid ulcers in s.c. injected tumors ?

Recently, we have injected s.c. B16F10 tumor cells in BL/6 mice. At around 12-15th day some animals showed ulcerations in tumors , with a big hole in the middle (it looks like a volcano). We have...

01 March 2021 9,938 3 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

What is the most suitable resuspension buffer for TCA/acetone precipitated protein?

I need to extract protein from fermented carbon sources for Bradford assay. Most researchers experiencing insolubility of pellet in resuspension buffer. Please assist me to select most suitable...

26 February 2021 7,129 3 View

How to plot the probability paper of Gumbel distribution using R programing?

In R-studio, there are many commands of Gumbel package. Arguments are also different. I`m asking about the alpha parameter of the Copula which must be greater than 1. If this is the one used to...

25 February 2021 5,229 3 View

Looking for genomic DNA extraction kits from mouse tissue (centrifuge-based, not spin-column)?

Hello. I am looking to extract genomic DNA from mouse brain and lungs and I need a kit that is not spin-column based because I need to scale up the solution amounts for 400mg tissue (most cap at...

25 February 2021 155 2 View

Edmund Pool

Check for inflammatory cytokines such as TNFa, IL-6 etc. Normally first to show up if there is an immune response.

Sarah Berndt

Hi Vamsi,

Happy to read your again...

I inject PLGA unloaded and PLGA loaded with lipids.

Thank you again. I will go for a Multiplex assay or CBA Assay (flow cytometry).

Rohulla Vaseq

hi sarah

can i know which rout do you use for injection?

Hi Ruhollah,

In the fat pads.

Mehdi Mahdavi

Hi Sarah ,

You can assay pro-inflammatory cytokines in the injection site via assay on tissue homogenates and also you can monitor inflammatory cell trafficking in this micro- environment via pathological smear.

Regards