20 June 2018 3 8K Report

We need to use best protocol to evaluate capacity of essential oil on shelf life of meat matrix as beef?

More Marius K. Somda's questions See All
Substitution YES/NO ?

Dear all, I'm wondering about a possible substitution of a G>A base or a deletion of the G base in view of the chromatograms I've analyzed. I would like to point out that I have observed the...

21 November 2023 8,904 2 View

How do I probe the interaction effect in a two-way repeated measures ANOVA?

Dear all, as part of my Master thesis, I am conducting a two-way repeated measures ANOVA on data from a social evaluation paradigm. The purpose for running this analysis is to investigate whether...

19 October 2023 3,746 1 View

What does theses chromatogrammes says?

Dear Sir/Madam, I am looking for a deletion or insertion anomaly in the CALR gene. After sequencing the same sample (Forward/Reverse), here's what I get. From the chromatograms we deduced that...

09 August 2023 4,907 4 View

BSC-DREAM8b-Dust model - restricted access?

I recently noticed that the BSC-DREAM8b-Dust model can no longer be accessed. http://www.bsc.es/ESS/bsc-dust-daily-forecast Do you have any idea if it can be accessed from another database or if...

11 March 2023 2,604 3 View

Can anyone help with the procedure to store fungal spores using glycerol?

I want to culture and store fungal spores of aspergillus using glycerol and need help with the procedure from anyone using it or have used it.

14 September 2022 9,087 3 View

How to induce HCC chemically to Wistar rats?

Hello scientists. I am seeking for natural compounds with anticancer activities. Actually I am interested to hepatocellular carcinoma (HCC). Unfortunately I am facing some issues to induce HCC...

09 June 2022 5,430 0 View

HELP ! CALR exon 9 Chromatogram analysis ?

Hello my Dear, I have a question about CALR chromatogram analysis. I sequenced and aligned the amplified sequences with the reference sequences but I'm having trouble identifying the anomalies...

13 April 2022 756 8 View

Differing morphology of Spirulina?

Hello, recently we have been working with the cyanobacterium Arthrospira platensis (Spirulina) and noticed, in shake flask culture cultured on an orbital shaker at 100 rpm, spherical cells and no...

01 February 2022 6,501 5 View

Immobilization of algae cells for cell counting?

Greetings, we are currently working on the cultivation of the microalgae Dunaliella tertiolecta. We try to count the cells in a Thoma cell counting chamber under the microscrope. Problem is, the...

10 August 2021 7,640 4 View

Improving spore production of Aspergillus oryzae?

Currently we are working on the cultivation of Aspergillus oryzae in shake flask liquid culture. To inoculate the cultures, a certain concentration of spores in the inoculum is wanted (Spore count...

11 March 2021 5,111 8 View

Similar questions and discussions
I need to dissolve stigmasterol for an animal experiment, it hasn't dissolved in either DMSO or vegetable oil. Can anyone recommend the best solvent?

Hello everyone, I need to dissolve stigmasterol for an animal experiment, but it hasn't dissolved in either DMSO or vegetable oil. Can anyone recommend the best solvent for stigmasterol?

01 August 2024 7,504 2 View

How to treat monolayer culture cell line with essential oil?

Hi all, may I ask you for advice about treating monolayer culture cell line with essential oil. Due to the hydrophobic character of the oil, I am concerning how can it equally affect to all the...

31 July 2024 5,301 2 View

Liposome Preparation using Heating Method - Why does my liposome suspension form aggregates?

Hello everyone, I am trying to encapsulate an essential oil in the liposomes using the heating method and encountering some issues. I am following the protocol described below, but my solution...

10 July 2024 4,083 3 View

How to dilute essential oil from plants to assess the biological activity?

I have extracted essential oil from a medicinal plant and want to make several concentrations for some biological activities (Antidiabetic assay). How can i prepare the several concentrations to...

04 July 2024 8,100 4 View

Alternative binders other than Nafion solution?

Hi Everyone, I plan to deposit a catalyst (TS-1@Co-PDA, in the core: TS-1 zeolite with a shell of Polydopamine designed with Cobalt) on a rotating ring-disk electrode (RRDE) to evaluate the...

29 June 2024 8,203 3 View

What are the causes for the non detection of Fatty acids peaks on a Rtxi 5MS column?

Dear scientific community, We used to performe many separations and detections on our GC MS (Perkin elmer Clarus 580-SQ8S), but recently we can not separate fatty acids( few ones appears in...

06 June 2024 6,844 0 View

Litopenaeus vannamei post-larvae for fatty acid and amino acid profile?

For fatty acid and amino acid profile analysis in Litopenaeus vannamei post-larvae (1-1.5 gr), is it necessary to remove the head and peel? or it's possible to use whole body including head, peel...

04 June 2024 4,497 0 View

What is the best buffer to resuspend proteins extracted from oil?

I hope everyone is well! I extracted proteins from sunflower and olive oils using acetone and then washing with n-hexane. I used RIPA buffer to resuspend because I plan to dot blot these samples....

02 June 2024 10,007 1 View

Assistance for hydrodistillation process?

I am a new researcher working on the extraction of essential oils from specific plants, starting with their leaves. However, I have encountered an issue with my Clevenger apparatus. Despite...

01 June 2024 2,445 7 View

How can I get an Annual Precipitation Data of a Province?

Hello forum members, I'm conducting research on vegetable oil fingerprinting. I need annual precipitation (rainfall) data for provinces in Thailand, Malaysia, and Indonesia. Specifically, I don't...

30 May 2024 6,209 2 View

Our partners will collect data and use cookies for ad personalization and measurement. Learn how we and our ad partner Google, collect and use data. Agree & close