Which analysis in gromacs can determine the validity of a defined topology and show the best topology among different topologies?

Hi , I am a beginner in gromacs. I have made some topology files for graphene sheet with different bonded and non-bonded parameters I found in the literature. Now, how can I choose the best...

08 May 2020 7,509 3 View

Recombinant protein production in insect cells using plasmid-based system?

Is it possible to produce a Flag-tagged catalytic domain of a murine enzyme in insect cells using a plasmid-based system? does it affect the functionality of the final enzyme?

29 November 2019 7,504 3 View

Hi dear researchers and professors can anybody help me?

Dear Researchers and Professors I like to write a review paper on multi-label classification as a conference paper, is anyone willing to cooperate? I can share the details of this...

11 December 2018 4,753 0 View

How can we get full text of articles which have no published PDF in the internet and have been published before 2000?

I am looking for some articles anywhere, searched internet, wrote letter to author, asked my professors for help, nevertheless couldn't get them. therefore I decide to test my chance here! Regards

05 June 2018 4,126 7 View

I need to some journals that publish the survey papers, please help! ?

I need to some journals that publish the survey papers, please help?!

04 May 2018 8,642 0 View

I need Scopus indexed journals list for computer science, is it available?

I need Scopus indexed journals list for computer science, is it available?

31 December 2017 6,049 3 View

As I decide to learn game theory in Python, please give me the best references, if possible?

As I decide to learn game theory in Python, please give me the best references, if possible?

08 September 2017 2,590 0 View

What is the difference between structured and unstructured mesh types in a FVM software ?

Hello, in a Finite Volume based software like Ansys Fluent, what is the difference between structured and unstructured mesh ? And what exactly are they ? I searched through the Internet, but...

15 August 2017 1,996 8 View

What is the connection between these issues "Bayesian Games Theory" and "Bayes Theorem"?

What is the connection between these issues "Bayesian Games Theory" and "Bayes Theorem"?

03 April 2017 5,021 0 View

Is it important correlation between labels in multi-label classification? why?

Is it important correlation between labels in multi-label classification?why?

21 March 2017 7,486 3 View

No title

02 March 2021 3,060 3 View

How to define and get results (stresses) for certain points of a model in abaqus, using python? When the size of the model changes?

I want to do a parameter study of a aixsymmetric cylinder in Abaqus. I want to create several jobs where I get the stresses for 3 different points in my model. These points are defined. I wrote a...

01 March 2021 10,078 1 View

LOAD DISPLACEMENT CURVES Stiffness increasing in ANSYS non linear analysis. HOW CAN I CORRECT IT?

Hi. I am doing non linear FE analysis using a new material beam under flexural 2 point loading using ANSYS. I have defined a bilinear material model. The solution has converged. In results I am...

01 March 2021 9,911 3 View

What are the countries most affected by extreme rainfall as a result of climate change?

Increased heat, drought and insect outbreaks, all associated to climate change, have increased wildfires. Decreasing water supplies, declined agricultural yields, health impacts in cities owing to...

01 March 2021 7,168 9 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View