I am using the pBAD24 arabinose inducible system in Shigella to express a protein of interest. My goal is to maintain induction of this protein during intracellular plaque assays. These assays require my mammalian cells to be plated 2 days prior to infection, then 3 days to allow for visible plaque formation. Thus, I need my cells to be glucose free for at least 5-6 days during my assay. Since this construct is repressed in the presence of glucose, I will not get any expression of my protein in typical cell culture media, which contains high glucose. Is it possible to grow mammalian cells without glucose?
Hello Jessica,
Mammalian cells needed basic media for its growth. As you didn't mention about the cell line types its difficult to say whether your cells will survive for such a long period without glucose. Generally Glioma Cells needed high glucose for their ideal growth. Cells which are grow in high glucose medium can sustain 24-36 hours without glucose, more the time u kept without glucose more the cells will be in stressed condition which is not recommended. So if you say the cell line or media which you used for your study i can suggest more accurately.
Good Luck
Prakash
Hi Prakash,
The cells are L2 mouse fibroblast cells. I have also tried supplementing my glucose free media with 1mM pyruvate and the cells also die.
Jessica
- Badges
- Science topic
- Similar topics
- Biological Science
- Cell Biology
- Culture Cells