What is the best method for separating wheat gluten protein subunits?

01 January 1970 6 5K Report

Hi to all

I'm going to work on variation in wheat gluten protein subunits (Gliadins and Glutenins) at protein and DNA levels. I would appreciate any advice in this regrad. for example: what is the best or the newest method for separating gluten protein subunits (Gliadins, HMW and LMW Glutenins)? or what are the possible problems in this way?

Thank you very much

Rajesh Kumar Gupta

Dear Sir,

Please find the attachment that will be help for you.

Thank you

Kayvan Agahi

Thank you very much Rajesh, it's really helpful.

Alaa Jabbar Al-Manhel

Hi Kayvan , you can see this link and attachment..good luck.

http://www.nature.com/articles/srep02279

Kayvan Agahi

Dear Alaa, thanks for your help brother

Salah Mahdi Najim

Dear Agahi,

Please check the following resources.

Best regards

SM Najim

http://www.aaccnet.org/publications/cc/backissues/1972/Documents/chem49_416.pdf

https://naldc.nal.usda.gov/download/31131/PDF

http://link.springer.com/protocol/10.1385%2F1-59259-082-9%3A055

http://www.brockwell-bake.org.uk/docs/Gluten_A%20Balance%20of%20Gliadin%20and%20Glutenin.pdf

http://ac.els-cdn.com/S1044030508005187/1-s2.0-S1044030508005187-main.pdf?_tid=06f46a62-b400-11e6-9b79-00000aab0f26&acdnat=1480182469_8818f4507034773d057b7e5a04d171ed

http://www.sciencedirect.com/science/article/pii/S0733521007000239

http://download.springer.com/static/pdf/118/art%253A10.1186%252F1477-5956-12-8.pdf?originUrl=http%3A%2F%2Fproteomesci.biomedcentral.com%2Farticle%2F10.1186%2F1477-5956-12-8&token2=exp=1480182673~acl=%2Fstatic%2Fpdf%2F118%2Fart%25253A10.1186%25252F1477-5956-12-8.pdf*~hmac=149b5b6f6b80139f87f13f8cebc805321b264e8ccd43da6e01d5cc2bd63cd62b

http://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0058682&type=printable

http://link.springer.com/article/10.1007/s11694-016-9345-9

Kayvan Agahi

Thanks thousands brother Salah

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