07 July 2018 3 3K Report

I wanna detection of IFN-gamma and IL4 from human tonsil what is the best method for protein detection ?

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Why are there multiple overexpressed protein bands in my SDS PAGE?

I have cloned a gene in Suffle T7 express strain using pET28a vector for expression and in vitro studies. After Ni-NTA column purification of this His Tagged protein, I am getting multiple bands...

01 February 2019 8,678 6 View

Can we use mixture of oligo dt and random hexamer for synthesis cDNA?

Is a random primer or oligo(dT) better for RT-PCR reaction? and Can we use them together

05 June 2018 7,563 3 View

How can I measure a gene with a very low expression by qRT PCR?

I wanna detection genes related to NKT cells (receptors), because of these cells are so low in our blood .detection of these genes is so hard and when I try by SYBR® Green Real-Time PCR, My...

05 June 2018 9,725 11 View

What is the reason for lines in separating gel?

I have a big problem with my SDS gel, When I run my proteins ,I see a line during running and after Staining and ,Destaining I have a line whole of my gel . I attach some photos of my gels.

05 June 2018 1,841 5 View

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