Hi there,

This should help:

http://onlinelibrary.wiley.com/doi/10.1016/j.femsle.2005.04.021/pdf

@ Dominique Liger: Thank u ..

Hi, 

you can try to isolate your protein using Mg/Chaps method or with Tris -EDTA method. If  you would not get your protein then firstly standardised your protein by enrichment. The paper linked below could help you at some extent.

@pritu pratibha: Thank u so much mam...Will try this..

Hi Poonam, see if this can be helpful - http://www.ncbi.nlm.nih.gov/pubmed/15927743

It varies fungus to fungus so it is important to know which one is your target fungus here