What is the best method for protein extraction from fungi (basidiomycota) for proteomics analysis??

More Poonam Kumari's questions See All

How do I perform antifungal susceptibilty using broth dilution for yeast?

Can anyone please provide me CLSI document M27-A3 ? I want to perform broth microdilution method for antifungal susceptibilty testing but do not know how to do it..Can anyone please explain it...

06 July 2015 3,978 4 View

Protein-aptamer gel electrophoresis help, please??

Hi, I have problems with running gel electrophoresis. I have tried agarose gel electrophoresis and native PAGE. I have two proteins, which have molecular weights of ~30kDa and ~180kDa and two...

03 March 2021 4,275 4 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

How to regulate SSCP temperature in simple PAGE?

I'd like to perform single-strand conformation polymorphism (SSCP) in my thesis, however I cannot control the temperature of the vertical PAGE since we are using the conventional tanks. Is there a...

02 March 2021 9,157 1 View

What is involved in morphological changes of SEM images of a substance?

In the sem analysis of CaCO3 nanaoparticles, the morphology images showed spherical shaped particles but the proposed morphology for these type of nanoparticles is trigonal. I prepared the...

01 March 2021 1,960 4 View

Why are my bands weird in this Agarose gel?

Can someone please give me some possible things that could go wrong? Here is my recipe: 0.5g Agarose 50 mL of TAE 1x 1 uL ethyl bromide. Gel was run at 100V for 1 hour. The buffer used is also TAE.

01 March 2021 9,952 3 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Isolates of Hymenoscyphus fraxineus?

Dear Dr. Cai, my name is Simone Prospero and I work in the team of Phytopathology at the Swiss Federal Institute for Forest Snow and Landscape Research (WSL;...

01 March 2021 1,133 1 View

Dominique Liger

Hi there,

This should help:

http://onlinelibrary.wiley.com/doi/10.1016/j.femsle.2005.04.021/pdf

Poonam Kumari

@ Dominique Liger: Thank u ..

Pritu Pratibha

Hi,

you can try to isolate your protein using Mg/Chaps method or with Tris -EDTA method. If you would not get your protein then firstly standardised your protein by enrichment. The paper linked below could help you at some extent.

@pritu pratibha: Thank u so much mam...Will try this..

Surya Kilaparty

Hi Poonam, see if this can be helpful - http://www.ncbi.nlm.nih.gov/pubmed/15927743

Subha Das

It varies fungus to fungus so it is important to know which one is your target fungus here