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Fermented beverages are very important drinks for some community as they consume daily with huge quantity, are traditionally believed as pharmacological and medicinal property along with...
16 June 2024 7,235 0 View
I have seeded HeLa cells in a calculation of 10000 cells per well in a 96 well plate. Then I went for treatment in the next day. Then at last, I added 7ul of 5mg/ml solution of MTT reagent into...
12 June 2024 1,460 3 View
I did GCMS/MS, LCMS of my sample of interest. I got Mass fragments in ionization process. Now facing issues to get the parent molecules name/formula. Due to complexity of sample (its a mixture of...
01 June 2024 1,056 4 View
please share the sources to download them
17 April 2024 5,244 0 View
Do I need to repeat the process or can I go for morphological/ Biochemical tests for fungi identification?
21 March 2024 5,153 1 View
I have done an RMSD validation for such protein by docking the two ligands one by one, but each docking results in large RMSD value compared to the reference.
22 January 2024 2,054 5 View
The presence or absence of sharp peaks in the diffraction graph of pure anthracene derivative versus thin films can be influenced by various factors. what are some potential reasons for the...
26 December 2023 1,176 1 View
how to confirmed the nanodiamonds using raman? how to distinguish carbon dots and nanodiamond?
18 December 2023 5,690 1 View
Can anyone suggest me how to plot the tafel from LSV data for CO2 REDUCTION?
05 November 2023 7,740 0 View
I am trying to extract lipids using water as solvent. After extraction followed by centrifugation, I did not obtain any oil layer. Instead the supernatant was milky white, which I think might be...
30 October 2023 9,151 1 View
Hello, I'm using the DynaPro NanoStar DLS system. I was wondering if anyone had previous experience in characterizing aggregates for adenovirus. I'm measuring samples as is, i.e undiluted. My...
26 September 2023 3,936 10 View
Hello Peers, Can anyone give me some resources or guidance on how to find the Refractive Index for Adenovirus ? Looking for any values previously reported in literature. Thank you in advance...
19 September 2023 2,024 6 View
Is it possible that in a co-infected host cell, DNA of a recombinant AAV integrates into the DNA of a wild-type adenovirus with non-homologous end-joining? Thus, may the wild-type adenovirus...
17 September 2023 4,670 3 View
Is it possible that a double-stranded DNA virus non-homologously recombine with a single-stranded DNA virus?
14 September 2023 7,342 2 View
Question about TEM images of VR-5 (Adenovirus A05) I attached my virus TEM images. I am confident that the particles in the image are adenovirus because the size of the particles is similar to...
16 July 2023 3,434 5 View
I am attempting to express a protein using an Adenovirus vector. I have successfully inserted the gene of interest into the Adenovirus and used linear pAdeno to transfect the 293A cell line....
29 March 2023 3,751 0 View
Hello, I am looking for a 3kb (approximate) stuffer sequence, I have seen that people use 'non-contiguous lambda bacteriophage DNA segments with no intact coding region' but I'm not too sure what...
29 November 2022 465 0 View
I have a mixture of adenovirus and AAV. I would like to remove the AAVs and leave only the adenovirus. Due to unique circumstances (that are unfortunately proprietary), I am unable to use affinity...
03 October 2022 6,531 2 View
Dear fellows, I am trying to produce and titrate Adenovirus-2 (VR-846 form ATCC) specifically on Vero cells. I had tested several protocols for this but it is not perfectly working. If possible,...
08 August 2022 9,437 2 View
Our goal is to engineer cells to express a synthetic receptor under the control of an endogenous promotor. We will therefore perform a CRISPR-Cas9 knock out (using RNPs) of the endogenous gene...
02 February 2022 9,556 0 View
