We are interested in the quantification of RNA from zika virus in the brain of embryos. We will use the RNeasy Lipid Tissue kit for extraction.
I will start to fabricate micro/nanoparticles for growth factor release and I check out a procedure quite nice, however, it is lacking some details and since I don't have much knowledge doing this...
20 February 2021 199 2 View
I am very new to quantitative PCR and need some help for this technique. For my experiment, I need to perform a quantitative reverse transcription PCR. I extract the RNA from my pellet and...
05 February 2021 9,834 4 View
I need to assimilate a determining condition for the plant. However, I need to ask you if it is possible to change the properties and concentration of plant nutrients in this material like perlite...
04 February 2021 8,616 11 View
I'm designing a low-grade chronic treatment with this cytokine and I'm wondering how regularly to change the culture medium based on the half-life of the protein in an in vitro environment....
24 January 2021 5,988 2 View
Tropidacris collaris is a grasshopper. Adults, which can reach more than 15 of length, are strong flyers and actually they resemble small birds or bats. Most of the time T. collaris is found in...
13 January 2021 3,735 5 View
Hello, I hope you are all doing well. I have a question regarding my treated cells group. I plan to do western blot and qRT-PCR for my treated cells. However, to save time, I would like to...
06 December 2020 1,390 5 View
Good day, I hope you are all doing well. I was designing primers for Caspase 9 and sometimes, the designed primers in NCBI have transcript variants for the same gene (Caspase 9) but at the same...
05 October 2020 352 3 View
Good day, I hope you are all well. I am new in designing primers for quantitative reverse transcription PCR. I am using NCBI to design my primers and my question is: Do I have to design my...
04 October 2020 7,678 7 View
Hello, I am studying the photophysical properties of a molecular rotor which unexcpectedly, shows a lambda(max, absorption) = 565 nm in toluene, but a lambda (max, absorption) = 540 nm in MeOH...
15 September 2020 7,830 3 View
Dear Colleagues, Please see the following link and information for more details. If you are thinking about submitting, please feel free to reach out to me directly here or at...
09 September 2020 6,361 1 View
I have selected brain tumor images ...but now found that already lots of research done n this topic.
03 March 2021 5,774 3 View
dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...
03 March 2021 6,994 5 View
I have created an Ice 1h crystalline structure and an Aluminium substrate structure and equilibrated both at 250K. Now I need to bring them together in a way that an adhesive bond is created....
01 March 2021 3,325 2 View
I need to model masonry and reinforced concrete minarets for my MSc thesis. Which one is easier and better at modeling slender masonry structures? ANSYS or ABAQUS? (Or any other program for just...
01 March 2021 736 3 View
There are different kinds of food stuffs which we intake daily according to our requirements. Carbohydrate, protein, fat, mineral, vitamin, water are mainly working for the growth and development...
01 March 2021 831 2 View
Millions ask how vaccines of covid 19 be successful and safe, its here to explain generally how vaccine of pfizer work ,mRNA vaccines teach our cells how to make a protein that will trigger an...
28 February 2021 2,723 4 View
I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...
28 February 2021 4,949 3 View
I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...
27 February 2021 356 6 View
The size of data generated by Ethovision is huge. Having NAS system for data storage seems to be a good option, I am wondering whether Ethovision can directly store the data to the NAS system...
27 February 2021 6,350 2 View
Warm greetings My name is Ayichew S. and I am doing my Ph.D. in Medical Microbiology (on Human papillomavirus). At this stage, I have almost completed a clinical sample Cervical swabs)...
25 February 2021 1,660 3 View