We are interested in the quantification of RNA from zika virus in the brain of embryos. We will use the RNeasy Lipid Tissue kit for extraction.
I am interested in publishing an updated version of my final master´s project in Spain and i am not sure which steps to take. I am open to suggestions.
16 July 2024 7,431 1 View
Have been trying to induce THP-1 using PMA at different concentrations (range from 5 to 50 ng/mL) but after 48h all the conditions except the control are mostly dead, checked by trypan blue and...
03 July 2024 3,884 5 View
Please can someone share the list of journals with impact factor published in 2024?
27 June 2024 2,927 0 View
I am working on a paper and need to calculate the q2 value in a model. However, I found that some of the values are negative. I am trying to interpret these negative values. Does anyone have any...
26 June 2024 1,176 0 View
I work in glycobiology and one of my duties is synthesize short oligosaccharide with, at least, one sugar universally 13C-labelled. Is there any sequence that can help me with characterization,...
28 May 2024 8,039 0 View
Hi! I left some petri dishes with Pseudomonas aeruginosa cultivated in MHI in the fridge (8°C) during some time off, and when I came back, it was like this. This is just one plate out of many, and...
24 May 2024 5,149 3 View
Estos articulos deben tener estas caracteristicas resumir los siguientes aspectos metodológicos: tipo de enfoque de investigación (cuantitativo, cualitativo o mixto), objetivo, hipótesis, muestra...
15 May 2024 820 0 View
Until the early 2000s, the National Bibliography used to be an important source for the development of library collections, mainly for the acquisition of new items. I would like to know if in your...
23 April 2024 5,037 7 View
For example: Episode 1 2 days duration Episode 2 4 days Ep 3 and son on Many thanks in advance
13 April 2024 4,735 0 View
VR or AR can be a useful tool to improve the learning experience of students, mainly in STEM topics or contents considering the complexity and abstraction need to think in analitycal mode, focused...
09 April 2024 739 2 View
I have virus (viral hemorrhagic septicemia virus) in suspension and the experiment will not involve cells. What level of TCID50 is preferred?
11 August 2024 3,115 1 View
Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...
11 August 2024 7,082 3 View
I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...
08 August 2024 9,882 2 View
I have modelled a steel structure using beam elements in Abaqus and attached to this structure reinforced concrete slab. The analysis that I am making is heat transfer of the structure. The...
07 August 2024 1,028 0 View
Brain and body mass together are positively correlated with lifespan (Hofman 1993). The duration of neural development is one of the best predictors of brain size, and conception is the best...
05 August 2024 6,247 3 View
I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...
05 August 2024 9,798 3 View
Hi everyone, I am working on brain slices for visualizing a protein in the soma and dendrites, using a fluorescence tag. However, I need a tool (not paid) for reconstruction of the whole neuron,...
04 August 2024 4,725 2 View
I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...
04 August 2024 501 2 View
I´ve been unable to find specific information about this neurotrophin in the CNS of rabbits exclusively. There is extensive info in mice, fish and rats, but in brain´s rabbit is hard to find....
04 August 2024 762 1 View
Do You Know Untreated TMJ Affects Your Brain And Entire Body?
04 August 2024 6,282 1 View